Speculations on the role of natural antisense transcripts in mammalian X chromosome evolution
Speculations on the role of natural antisense transcripts in mammalian X chromosome evolution
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DOI：
10.1159/000071587
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发表时间：
2002-01-01
影响因子：
1.7
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Kiyosawa, H;Abe, K
最近在小鼠中进行的全面的转录组分析揭示了在真核生物中迄今为止被忽视的一类基因中存在大量的天然反义转录物。我们讨论了这些转录本可能的生物学作用及其与哺乳动物性染色体进化的关系。在60，770个全长cDNA序列中，已经鉴定了多达2，500对有义-反义转录物（SAT），其具有通过其互补序列形成RNA双链体的潜力。这种高数量的反义转录物表明它们在基因表达调控中的一般作用。除了X染色体外，这些SAT几乎沿着染色体均匀分布。X染色体上SAT的发生率是常染色体的三分之一到二分之一，这种代表性不足一定与X染色体固有的特性有关。在这里，我们假设，monoallelically表达的反义RNA调节其正义的合作伙伴，但这种调节系统不能在哺乳动物的X染色体上操作，因为哺乳动物的X染色体是有效的在两性的半合子状态。这种调控的丧失可能与X染色体本身的进化有关。版权所有（C）2002 S. Karger AG，巴塞尔。
Recent comprehensive transcriptome analyses in mice have revealed tremendous numbers of natural antisense transcripts in a hitherto ignored category of genes in eukaryotes. We discuss the possible biological roles of these transcripts and their relationships with mammalian sex chromosome evolution. Of 60,770 full-length cDNA sequences, as many as 2,500 pairs of sense-antisense transcripts (SATs) with the potential to form RNA duplex via their complementary sequences have been identified. This high number of antisense transcripts indicates their generic roles in gene expression regulation. These SATs are almost evenly distributed along the chromosomes, with the exception of the X chromosome. The rate of occurrence of SATs on the X chromosome is one-third to one-half that on the autosomes, and this under-representation must be related to a property intrinsic to the X chromosome. Here we hypothesize that monoallelically expressed antisense RNA regulates its sense partner, but that this regulatory system cannot operate on the mammalian X chromosome, as the mammalian X chromosome is effectively in a hemizygous state in both sexes. Loss of such regulation may be involved in the evolution of the X chromosome itself. Copyright (C) 2002 S. Karger AG, Basel.