Measurement of Ether Phospholipids in Human Plasma with HPLC-ELSD and LC/ESI-MS After Hydrolysis of Plasma with Phospholipase A1.
Measurement of Ether Phospholipids in Human Plasma with HPLC-ELSD and LC/ESI-MS After Hydrolysis of Plasma with Phospholipase A1.
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DOI：
10.1007/s11745-016-4170-9
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发表时间：
2016-08
期刊：
影响因子：
1.9
通讯作者：
Fujino T
中科院分区：
文献类型：
--
作者：
Mawatari S;Hazeyama S;Fujino T
乙醇胺醚磷脂（eEtnGpl）和胆碱醚磷脂（eChoGpl）存在于人血浆或血清中，但血浆中醚磷脂的相对浓度与其他组织中的相对浓度相比非常低。目前，血浆中醚磷脂的测量依赖于串联质谱法（LC/MS/MS），但是LC/MS/MS系统对于通常的临床实验室来说通常太昂贵。用磷脂酶A1（PLA 1）处理血浆会导致二酰基磷脂完全水解，但醚磷脂保持完整。在用PLA 1处理血浆后，通过高效液相色谱蒸发光散射检测（HPLC-ELSD）检测eEtnGpl和eChoGpl作为独立峰。用于HPLC-ELSD的相同样品可用于用电喷雾电离质谱法检测eEtnGpl和eChoGpl。人血浆中eChoGpl和eEtnGpl两者中烷基酰基磷脂的存在通过用PLA 1和盐酸连续水解血浆来指示。
Ethanolamine ether phospholipid (eEtnGpl) and choline ether phospholipid (eChoGpl) are present in human plasma or serum, but the relative concentration of the ether phospholipids in plasma is very low as compared to those in other tissues. Nowadays, measurement of ether phospholipids in plasma depends on tandem mass spectrometry (LC/MS/MS), but a system for LC/MS/MS is generally too expensive for usual clinical laboratories. Treatment of plasma with phospholipase A1 (PLA1) causes complete hydrolysis of diacylphospholipids, but ether phospholipids remain intact. After the treatment of plasma with PLA1, both eEtnGpl and eChoGpl are detected as independent peaks by high-performance liquid chromatography with evaporative light scattering detection (HPLC–ELSD). The same sample used for HPLC–ELSD can be applied to detect eEtnGpl and eChoGpl with electrospray ionization mass spectrometry. Presence of alkylacylphospholipids in both eChoGpl and eEtnGpl in human plasma was indicated by sequential hydrolysis of plasma with PLA1 and hydrochloric acid.