Multiple sequence elements are required for regulation of human T-cell leukemia virus gene expression.
Multiple sequence elements are required for regulation of human T-cell leukemia virus gene expression.
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人类T细胞白血病病毒基因表达的调节需要多个序列元件。
DOI：
10.1073/pnas.84.14.4919
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发表时间：
1987
影响因子：
11.1
通讯作者：
Haseltine,WA
中科院分区：
文献类型：
--
作者：
Rosen,CA;Park,R;Sodroski,JG;Haseltine,WA
人类 T 细胞白血病病毒 I 型 (HTLV-I) 长末端重复 (LTR) 的 U3 区域包含对原病毒 pX 区域编码的反式激活转录 (tat) 因子作出反应的序列。这里给出的结果表明，LTR 内存在多个 tat 响应序列，并且在 U3 区域内重复 3 次的单个 21 核苷酸序列足以确定对反式激活剂的响应。该序列能够赋予HTLV-I和猿猴病毒40启动子tat响应表型，与方向无关。有效HTLV-1 LTR指导的基因表达所需的序列也位于RNA起始位点的3'，在LTR的R和U5区域内。
The U3 region of the long terminal repeat (LTR) of human T-cell leukemia virus type I (HTLV-I) contains sequences that respond to the trans-activating transcription (tat) factor encoded by the pX region of the provirus. Results presented here show that there are multiple tat-responsive sequences within the LTR and that a single 21-nucleotide sequence, which is repeated three times within the U3 region, is sufficient to determine the response to the trans-activator. This sequence is capable of conferring a tat-responsive phenotype upon the HTLV-I and simian virus 40 promoters, independent of orientation. Sequences required for efficient HTLV-I LTR-directed gene expression are also located 3' to the site of RNA initiation, within the R and U5 regions of the LTR.