Integrated aerial and destructive phenotyping differentiates chilling stress tolerance during early seedling growth in sorghum
Integrated aerial and destructive phenotyping differentiates chilling stress tolerance during early seedling growth in sorghum
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DOI：
10.1016/j.fcr.2018.07.011
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发表时间：
2018-10-01
影响因子：
5.8
通讯作者：
Jagadish, S. V. Krishna
中科院分区：
文献类型：
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作者：
Chiluwal, Anuj;Bheemanahalli, Raju;Jagadish, S. V. Krishna
早季抗寒性较强的高粱有可能减少终端干旱和热胁迫的损害，并有效地利用早季残留水分。以4个有希望的耐冷高粱高代育种系、一个已知的早期耐冷的中国地方品种（山葵红- SQR）和一个感冷的美国优良品种（RTx 430）为对照，在田间和受控环境下评估了出苗和早期生长期间的耐冷性。在两个独立的受控环境生长室实验中，将植物暴露于6/6 ° C、8/8 ° C、10/10 ° C、15/15 ° C、20/10 ° C、20/15 ° C和32/22 ° C的最大白天/最小夜间温度。在2016年进行了两次早期（4月1日和15日）和定期（5月13日和6月2日）种植的田间研究，并在2017年进行了一次早期（4月21日）和定期（5月25日）种植。出苗率和出苗后一个月收获的幼苗，叶面积，地上部重量，根重量，和其他根形态性状记录从所有实验。利用配备多光谱相机的无人机进行空中表型分析，以获取基于反射率的植被指数（NDVI和NDRE）。在低温条件下，部分优良品系的出苗率、幼苗生长势、根系生物量均显著优于SQR。航空表型指数从30和60天之间出现后拍摄的图像一致相关的破坏性测量下早期种植，表明其有效性，在区分低温反应。气生表型指标可有效地用于高粱遗传多样性群体的表型分析，以提高早季高粱的耐冷性。
Sorghum with greater chilling tolerance during early season has the potential to minimize terminal drought and heat stress damage and efficiently utilize early season residual moisture. Four promising chilling stress tolerant sorghum advanced breeding lines, a known early stage chilling tolerant Chinese landrace (Shan Qui Red - SQR) and a susceptible US elite cultivar (RTx430) as checks were assessed for chilling tolerance during emergence and early growth under field and controlled environments. Plants were exposed to a maximum day-time/minimum night-time temperature of 6/6 degrees, 8/8 degrees, 10/10 degrees, 15/15 degrees, 20/10 degrees, 20/15 degrees and 32/22 degrees C in two independent controlled environment growth chamber experiments. Field studies were conducted with two early (April 01 and 15) and regular (May 13 and June 02) plantings in 2016 and with one early (April 21) and regular (May 25) planting in 2017. Emergence rate and from seedlings harvested one month after emergence, leaf area, shoot weight, root weight, and other root morphological traits were recorded from all experiments. Aerial phenotyping using UAV fitted with multispectral camera was used to capture reflectance-based vegetation indices (NDVI and NDRE). Some advanced breeding lines recorded significantly better emergence, seedling growth and vigor, root biomass compared to SQR under chilling conditions. Aerial phenotyping indices from images taken between 30 and 60 days after emergence were consistently correlated with destructive measurements under early plantings, indicating their effectiveness in differentiating chilling responses. Aerial phenotyping indices have the potential to be utilized effectively for phenotyping genetically diverse populations for improving early season chilling tolerance in sorghum.