COPPER METALLOTHIONEIN OF YEAST, STRUCTURE OF THE GENE, AND REGULATION OF EXPRESSION
COPPER METALLOTHIONEIN OF YEAST, STRUCTURE OF THE GENE, AND REGULATION OF EXPRESSION
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DOI：
10.1073/pnas.81.11.3332
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
CROOKE, ST
中科院分区：
文献类型：
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作者：
BUTT, TR;STERNBERG, EJ;CROOKE, ST
在酵母 [Saccharomyces cerevisiae] 细胞中添加 Cu 会诱导产生一种低分子量、富含半胱氨酸的蛋白质，该蛋白质与 Cu 结合。这种蛋白质被称为铜螯合蛋白或硫因，与脊椎动物细胞中响应镉和锌而诱导的金属硫蛋白家族相关。酵母铜结合蛋白的结构通过基因的DNA序列分析来确定。尽管 6573 道尔顿酵母蛋白与脊椎动物金属硫蛋白有很大不同，但作为金属结合蛋白标志的 12 个半胱氨酸残基的排列是部分保守的。通过将该基因与大肠杆菌半乳糖激酶基因融合并测定酵母中响应铜的酶活性水平来分析该基因的调控DNA序列。转录激活对铜有特定的要求。 Zn、Cd 和 Au 不能调节半乳糖激酶活性。酵母铜金属硫蛋白调控序列代表了一类以前未报道的受 Cu 调控的酵母启动子。
Addition of Cu to yeast [Saccharomyces cerevisiae] cells leads to the induction of a low MW, cysteine-rich protein that binds Cu. This protein, termed copper chelatin or thionein, is related to the metallothionein family of proteins that are induced in response to Cd and Zn in vertebrate cells. Structure of the yeast Cu-binding protein was determined by DNA sequence analysis of the gene. Although the 6573 dalton yeast protein is substantially divergent from vertebrate metallothioneins, the arrangement of 12 cysteine residues, which is a hallmark of metal-binding proteins, is partially conserved. The regulatory DNA sequence of the gene was analyzed by fusing it with the Escherichia coli galactokinase gene and assaying the levels of enzyme activity in yeast in response to Cu. Transcriptional activation has a specific requirement for Cu. Zn, Cd and Au were unable to regulate the galactokinase activity. The yeast copper metallothionein regulatory sequences represent a previously unreported class of yeast promoter that is regulated by Cu.