Evidence for expression of estrogen receptor cofactor messenger ribonucleic acid in the ovary and uterus of domesticated animals (sheep, cow and pig).
Evidence for expression of estrogen receptor cofactor messenger ribonucleic acid in the ovary and uterus of domesticated animals (sheep, cow and pig).
复制标题
家养动物（绵羊、牛和猪）的卵巢和子宫中雌激素受体辅因子信使核糖核酸表达的证据。
DOI：
10.1016/s0024-3205(01)00937-7
复制
发表时间：
2001
期刊：
影响因子：
6.1
通讯作者：
Nephew,KP
中科院分区：
文献类型：
--
作者：
Hlaing,M;Nam,K;Lou,J;Pope,WF;Nephew,KP
雌激素受体辅因子（辅激活因子或辅抑制因子）在特定组织区室和细胞中的表达水平被认为影响雌激素应答基因的表达，从而影响靶组织的总体激素应答。迄今为止，已报道在人、大鼠和小鼠的组织中存在辅因子。我们分析了信使核糖核酸（mRNA）编码的几个转录辅因子的存在和分布在卵巢和子宫的三个家畜物种，羊，牛和猪。辅因子SRC-1、GRIP 1、RAC 3、p300、RIP 140和SPA的北方分析显示在所有三个物种的卵巢中表达。此外，在黄体期（发情周期的第10-12天）观察到SRC-1、GRIP 1、RAC 3、p300和RIP 140 mRNA的表达低于发情期（第0天）;然而，SPA转录水平保持不变。然后，我们研究了绵羊卵巢中变化（SRC-1，RIP 140）和组成型表达（SPA）辅因子的mRNA表达。与卵巢周围组织相比，黄体中SRC-1和RIP 140的转录水平较低。SPA mRNA的表达，然而，在黄体和周围组织中是相似的。为了确定哪些卵巢细胞类型表达SRC-1、RIP 140和SPA，在绵羊卵巢上进行原位杂交。对应于这些辅因子的银颗粒可见于卵巢颗粒、卵泡膜和基质细胞，但似乎在颗粒细胞中最丰富。然而，与卵泡细胞中的表达相比，SRC-1和RIP 140在黄体中的表达减少。最后，我们研究了辅因子在绵羊、牛和猪子宫中的表达。SRC-1、GRIP 1、RAC 3、p300和RIP 140 mRNA的北方印迹分析显示，与整个子宫相比，子宫内膜提取物中的表达更高。我们提供了第一个证据，雌激素受体辅因子mRNA的存在下，在卵巢和子宫的三个家养动物物种。我们认为，辅激活因子是保守的物种之间，并与生殖道组织的激素反应在羊，牛和猪。
Expression levels of estrogen receptor cofactors (coactivators or corepressors) in specific tissue compartments and cells are thought to influence the expression of estrogen responsive genes and thereby influence overall hormonal responsiveness of target tissues. To date, the presence of cofactors has been reported in tissues from humans, rats and mice. We analyzed the presence and distribution of messenger ribonucleic acids (mRNAs) encoding several transcriptional cofactors in the ovary and uterus of three domestic animal species, the sheep, cow and pig. Northern analysis for cofactors SRC-1, GRIP1, RAC3, p300, RIP140, and SPA showed expression in ovaries from all three species. In addition, lower expression of SRC-1, GRIP1, RAC3, p300, and RIP140 mRNAs was observed during the luteal phase (day 10–12 of the estrous cycle) than at estrus (day 0); however, SPA transcript levels remained unchanged. We then examined expression of mRNAs for changing (SRC-1, RIP140) and constitutively expressed (SPA) cofactors in ovine ovaries. SRC-1 and RIP140 transcripts in corpus luteum were lower compared to the surrounding ovarian tissue. SPA mRNA expression, however, was similar in corpus luteum and surrounding tissues. To determine which ovarian cell types express SRC-1, RIP140, and SPA, in situ hybridization was performed on sheep ovaries. Silver grains corresponding to these cofactors were seen in ovarian granulosa, theca and stromal cells, but appeared to be most abundant in the granulosa cells. Expression of SRC-1 and RIP140 in corpus luteum, however, was reduced compared to expression in follicular cells. Finally, we examined cofactor expression in ovine, bovine, and porcine uterus. Northern blot analysis for SRC-1, GRIP1, RAC3, p300, and RIP140 mRNAs showed higher expression in extracts of the endometrium compared to whole uterus. We provide the first evidence for the presence of estrogen receptor cofactor mRNAs in the ovary and uterus of three domestic animal species. We suggest that coactivators are conserved among species and associated with hormonal responsiveness of reproductive tract tissues in sheep, cow and pig.