Phenotype and hematopoietic potential of side population cells throughout embryonic development
Phenotype and hematopoietic potential of side population cells throughout embryonic development
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DOI：
10.1182/blood-2003-01-0118
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发表时间：
2003-10-01
期刊：
影响因子：
20.3
通讯作者：
Hirschi, KK
中科院分区：
文献类型：
--
作者：
Nadin, BM;Goodell, MA;Hirschi, KK
采用Hoechst 33342-挤压侧群（SP）细胞分选纯化成鼠骨髓造血干细胞（hsc）。在此，我们研究了SO细胞是否存在于胚胎组织中并表现出造血祖细胞活性。我们分离了交尾后7.5 ~ 11.5天的卵黄囊（YS）和胚胎组织（dpc），分别分离了一个SP细胞，发现这些SP细胞在整个发育过程中表现出不同的表型和功能特征。YS和胚胎SP分离的8.0 dpc表达血管内皮-cadherin （VE-cadherin）和血管内皮受体2 (Flk-1)，这些标记物不是在骨髓SP中表达，而是在内皮细胞和祖细胞中表达。这一阶段的SP在体外不表达CD45或产生造血集落。相比之下，SP分离9.5至11.5 dpc含有显著更高比例的表达cKit和CD45的细胞，这些标记物在骨髓SP中高度表达。此外，YS SP分离9.5至11.5 dpc的造血祖细胞活性比未分离的组织高40至90倍。我们的数据表明，在循环开始之前检测到的YS和胚胎SP细胞表达最高水平的内皮标志物，并且在体外不产生血细胞；然而，随着发育的进展，它们获得了与骨髓SP相似的造血潜能和表型特征。(C) 2003年由美国血液学学会公布。
Adult murine bone marrow hematopoietic stem cells (HSCs) can be purified by sorting Hoechst 33342-extruding side population (SP) cells. Herein we investigated whether SO cells reside within embryonic tissues and exhibit hematopoietic progenitor activity. We isolated yolk sac (YS) and embryonic tissues 7.5 to 11.5 days after coitus (dpc), resolved an SP in each, and demonstrated that these SP cells exhibit distinct phenotypic and functional characteristics throughout development. YS and embryonic SP isolated 8.0 dpc expressed vascular endothelial-cadherin (VE-cadherin) and vascular endothelial receptor 2 (Flk-1), markers not expressed by bone marrow SP but expressed by endothelial cells and progenitors. SP at this stage did not express CD45 or produce hematopoietic colonies in vitro. In contrast, SP isolated 9.5 to 11.5 dpc contained a significantly higher proportion of cells expressing cKit and CD45, markers highly expressed by bone marrow SP. Furthermore, YS SP isolated 9.5 to 11.5 dpc demonstrated 40- to 90-fold enrichment for hematopoietic progenitor activity over unfractionated tissue. Our data indicate that YS and embryonic SP cells detected prior to the onset of circulation express the highest levels of endothelial markers and do not generate blood cells in vitro; however, as development progresses, they acquire hematopoietic potential and phenotypic characteristics similar to those of bone marrow SP. (C) 2003 by The American Society of Hematology.