Vaccination with a Paramyosin-Based Multi-Epitope Vaccine Elicits Significant Protective Immunity against Trichinella spiralis Infection in Mice.
Vaccination with a Paramyosin-Based Multi-Epitope Vaccine Elicits Significant Protective Immunity against Trichinella spiralis Infection in Mice.
复制标题
接种基于副肌球蛋白的多表位疫苗可在小鼠体内引发针对旋毛虫感染的显着保护性免疫。
DOI：
10.3389/fmicb.2017.01475
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Gu Y;Sun X;Li B;Huang J;Zhan B;Zhu X
旋毛虫病是一种世界性的人畜共患病，是一个严重的公共卫生问题。通过对家畜接种强效疫苗阻断寄生虫传播是预防人类旋毛虫病的一种实用方法。我们前期的研究表明旋毛虫副肌球蛋白（Ts-Pmy）是一种很好的旋毛虫病疫苗候选物。本研究利用Ts-Pmy的4个CD 4 + T细胞表位（P2、P3、P4和P5）和1个B细胞表位（YX 1）构建了一种新型的多表位疫苗（MEP），并在大肠杆菌中表达了可溶性重组蛋白（rMEP）。rMEP疫苗免疫小鼠，肌幼虫减少率（55.4%）显著高于亲本rTs-Pmy疫苗免疫小鼠（34.4%）。螺旋体感染rMEP诱导的高水平抗rMEP特异性IgG和IgG 1/IgG 2a亚类抗体、脾细胞T细胞增殖以及IFN-γ、IL-4和IL-5的分泌均与较好的保护作用有关。对单个T细胞表位的细胞应答也表明，rMEP免疫小鼠的脾细胞对合成表位肽P2、P3和P4的刺激有强烈的应答，但对P5没有应答，这表明大多数T细胞表位在免疫过程中暴露并被良好地加工，这可能有助于rMEP免疫诱导的高保护性。本研究表明，表位疫苗是一种很有前途的旋毛虫病疫苗研究方法。
Trichinellosis is a worldwide zoonosis and remains a serious public health problem. Interrupting parasite transmission via vaccination of livestocks with a potent vaccine is a practical approach to prevent human Trichinellosis. Our previous studies have identified that paramyosin of Trichinella spiralis (Ts-Pmy) is a good vaccine candidate against Trichinellosis. In this study, a novel multi-epitope vaccine (MEP) was constructed by using four CD4+ T cell epitopes (P2, P3, P4, and P5) and one B cell epitope (YX1) from Ts-Pmy and expressed as a soluble recombinant protein (rMEP) in Escherichia coli. Mice immunized with rMEP vaccine produced significant higher muscle larval reduction (55.4%) than that induced by immunization of parental rTs-Pmy (34.4%) against T. spiralis infection. The better protection is associated with rMEP induced high levels of anti-rMEP specific IgG and subclass IgG1/IgG2a, elevated T cell proliferation of splenocytes and secretion of IFN-γ, IL-4 and IL-5. The cellular response to individual T cell epitope also showed that splenocytes from mice immunized with rMEP strongly response to the stimulation of synthetic epitope peptide P2, P3, and P4, but not to P5, suggesting that most of T cell epitopes are exposed and processed well during immunization that may contribute to the high protection induced by the immunization of rMEP. This study implies that epitope vaccine is a promising approach for the development of vaccines against Trichinellosis.