N-cadherin mediates cortical organization in the mouse brain
N-cadherin mediates cortical organization in the mouse brain
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DOI：
10.1016/j.ydbio.2006.12.014
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发表时间：
2007-04-01
影响因子：
2.7
通讯作者：
Takeichi, Masatoshi
中科院分区：
文献类型：
--
作者：
Kadowaki, Masakazu;Nakamura, Shoko;Takeichi, Masatoshi
大脑皮层是一个复杂的层状结构，由发育中的神经元从心室区连续迁移而产生。其中一个可能在皮质形态发生中起作用的分子是n -钙粘蛋白，因为它的阻断会导致其他神经组织（如神经视网膜）细胞有序排列的破坏。在这里，我们发现当n -钙粘蛋白基因在小鼠大脑皮层中被有条件地删除时，皮质内结构几乎完全随机化；例如，有丝分裂细胞和有丝分裂后细胞无顺序地分散在皮层中。这些缺陷似乎主要源于位于神经上皮细胞顶端的粘附连接（AJs）的破坏，而n -钙粘蛋白通常在这里最高浓度。在缺乏n -钙粘蛋白的情况下，神经上皮细胞或径向胶质细胞不能扩展其体或突以跨越脑室和脑脊膜表面之间的距离，因此终止于皮层的中间区域。这些结果表明，n -钙粘蛋白对于维持神经上皮细胞或放射状胶质细胞的正常结构至关重要，它们的破坏使皮层的内部结构随机化。(c) 2006爱思唯尔公司版权所有。
The cerebral cortex is a complex laminated structure generated by the sequential migration of developing neurons from the ventricular zone. One of the molecules that may play a role in cortical morphogenesis is N-cadherin since its blocking causes disruption of the ordered arrangement of cells in other neural tissues, such as the neural retina. Here, we show that when the N-cadherin gene had been conditionally deleted in the mouse cerebral cortex, the intra-cortical structures were nearly completely randomized; e.g., mitotic cells and postmitotic cells were scattered throughout the cortex without any order. These defects seemed to mainly originate from the disruption of the adherens junctions (AJs) localized in the apical end of neuroepithelial cells, where N-cadherin is normally most highly concentrated. In the absence of N-cadherin, neuroepithelial or radial glial cells could not expand their bodies or processes to span the distance between the ventricular and pial surfaces and therefore terminated them in the middle zone of the cortex. These results demonstrate that N-cadherin is essential for maintaining the normal architecture of neuroepithelial or radial glial cells and that their disruption randomizes the internal structures of the cortex. (c) 2006 Elsevier Inc. All rights reserved.