Low-solubility glycerol dehydratase, a chimeric enzyme of coenzyme B12-dependent glycerol and diol dehydratases
Low-solubility glycerol dehydratase, a chimeric enzyme of coenzyme B12-dependent glycerol and diol dehydratases
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低溶解度甘油脱水酶，辅酶 B12 依赖性甘油和二醇脱水酶的嵌合酶
DOI：
10.1007/s00203-008-0443-x
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发表时间：
2009
影响因子：
2.8
通讯作者：
T. Toraya
中科院分区：
文献类型：
--
作者：
T. Tobimatsu;Tsuneo Nishiki;Masaya Morimoto;Ryou Miyata;T. Toraya
辅酶b12依赖的二醇和甘油脱水酶是同功能酶，催化1,2 -二醇脱水生成相应的醛。虽然这两种类型的脱水酶具有高度的序列同源性，但甘油脱水酶是一种可溶性胞质酶，而二醇脱水酶是一种与羧酶样多面体细胞器相关的低溶解度酶。由于β亚基的n端20和γ亚基的16个氨基酸残基对二醇脱水酶的低溶解度都是必不可少的，我们构建了基于甘油脱水酶的嵌合酶，在相应的甘油脱水酶亚基中携带二醇脱水酶的β和γ亚基的n端部分。在二醇脱水酶的n端分别加入β亚基34和γ亚基33个氨基酸残基并不足以降低甘油脱水酶的溶解度。一种嵌合酶携带二醇脱水酶β亚基的低同源区（残基35-60）以及二醇脱水酶特异性的β和γ亚基外区，其溶解度与二醇脱水酶相当，尽管其亲水性图在这些区域没有显示出任何明显的疏水峰。由此得出结论，短n端序列足以改变酶的溶解度。
Coenzyme B12-dependent diol and glycerol dehydratases are isofunctional enzymes, which catalyze dehydration of 1, 2-diols to produce corresponding aldehydes. Although the two types of dehydratases have high sequence homology, glycerol dehydratase is a soluble cytosolic enzyme, whereas diol dehydratase is a low-solubility enzyme associated with carboxysome-like polyhedral organelles. Since both the N-terminal 20 and 16 amino acid residues of the β and γ subunits, respectively, are indispensable for the low solubility of diol dehydratase, we constructed glycerol dehydratase-based chimeric enzymes which carried N-terminal portions of the β and γ subunits of diol dehydratase in the corresponding subunits of glycerol dehydratase. Addition of the diol dehydratase-specific N-terminal 34 and 33 amino acid residues of the β and γ subunits, respectively, was not enough to lower the solubility of glycerol dehydratase. A chimeric enzyme which carries the low homology region (residues 35–60) of the diol dehydratase β subunit in addition to the diol dehydratase-specific extra-regions of β and γ subunits showed low solubility comparable to diol dehydratase, although its hydropathy plot does not show any prominent hydrophobic peaks in these regions. It was thus concluded that short N-terminal sequences are sufficient to change the solubility of the enzyme.
丙二醇脱水酶（一种膜相关酶）的纯化和亚基表征。
影响因子：
2.9
作者：
McGee,DE;Richards,JH
通讯作者：
Richards,JH
影响因子：
2.9
作者：
GILL, SC;VONHIPPEL, PH
通讯作者：
VONHIPPEL, PH