Collagen-based modified membranes for tissue engineering: Influence of type and molecular weight of GAGs on cell proliferation
Collagen-based modified membranes for tissue engineering: Influence of type and molecular weight of GAGs on cell proliferation
复制标题
DOI：
10.1016/j.ijpharm.2009.05.049
复制
发表时间：
2009-08-13
影响因子：
5.8
通讯作者：
Vandelli, Maria Angela
中科院分区：
文献类型：
--
作者：
Ruozi, Barbara;Parma, Bruna;Vandelli, Maria Angela
本研究旨在评价两种应用最广泛的糖胺多聚糖(硫酸皮肤素和肝素)对用于组织工程设计的胶原基修饰膜(COL/GAGS膜)结构和生物学性能的影响。硫酸皮肤素和肝素的相对分子质量与膜的可行性和细胞(成纤维细胞和角质形成细胞)在Col/GAG膜上的黏附和增殖能力有关。用扫描电子显微镜和差示扫描量热法研究了Col/GAG膜的微观结构和理化性能，并测定了膜中游离氨基的含量和溶胀性能。用光镜和比色法观察角质形成细胞和成纤维细胞的形态、增殖和生长行为。成纤维细胞和角质形成细胞都能在胶原/硫酸皮肤素复合膜上生长和增殖。与未加肝素的膜(COL膜)相比，含肝素的膜上成纤维细胞的增殖明显增强。特别是添加高分子量硫酸皮肤素和高分子量肝素的复合膜适合作为生物材料作为表皮替代物。(C)2009爱思唯尔B.V.保留所有权利。
This study aims to evaluate the effects of the two most widely used glycosaminoglycans (dermatan sulphate and heparin) on both the structural and biological properties of collagen-based modified membranes (COL/GAGs membranes) designed for tissue engineering. The molecular weight of dermatan sulphate and heparins was correlated with the membrane feasibility and the cell (fibroblasts and keratinocytes) ability to adhere and proliferate on the COL/GAG membranes.Microstructure and physico-chemical properties of COL/GAGs membranes were examined using scanning electron microscopy and differential scanning calorimetry; the free amino group content and the swelling properties were also detected. The morphology, proliferation and growth behaviour of keratinocytes and fibroblasts were investigated using microscopical approach and in vitro colorimetric assay. Both fibroblasts and keratinocytes are able to grow and proliferate on COL/dermatan sulphate membranes. Fibroblasts revealed significantly higher proliferation on the membranes prepared with heparin if compared to the proliferation on the membrane without heparin (COL membrane). Particularly, a combination of the membranes formulated adding high molecular weight dermatan sulphate and high molecular weight heparin could be suitable to be used as biomaterials for epidermal substitute. (C) 2009 Elsevier B.V. All rights reserved.