Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy
Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy
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通过 GradedPool-Seq 图谱剖析杂种优势基因为水稻改良策略提供信息
DOI：
10.1038/s41467-019-11017-y
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发表时间：
2019-07-05
影响因子：
16.6
通讯作者：
Han, Bin
中科院分区：
文献类型：
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作者：
Wang, Changsheng;Tang, Shican;Han, Bin
杂交水稻育种利用杂种优势、杂种优势，极大地提高了粮食产量。然而，与水稻籽粒产量相关的杂种优势相关基因在很大程度上仍然未知，部分原因是杂种优势相关性状的综合定位仍然是劳动密集型和耗时的。本文提出了一种利用F2代分级池样品的全基因组测序快速定位QTL的方法GradedPool-Seq。利用该方法和图位克隆技术，从母本中分离出了具有杂种优势的QTLGW 3 p6。将母本广湛63 -4S的GW 3 p6等位基因导入父本辐恢676，获得近等基因系NIL-FH 676：：GW 3 p6。与福恢676相比，NIL-FH 676：：GW 3 p6表现出显著增加的籽粒产量。这项研究表明，它可能是可能的，以实现高水平的粮食生产的自交系，而不需要构建杂交种。
Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of graded-pool samples from F2progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTLGW3p6from the female line. We then generate the near isogenic line NIL-FH676::GW3p6by introgressing theGW3p6allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676::GW3p6exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.