QTL Mapping of Seed Vigor of Backcross Inbred Lines Derived From Oryza longistaminata Under Artificial Aging
QTL Mapping of Seed Vigor of Backcross Inbred Lines Derived From Oryza longistaminata Under Artificial Aging
复制标题
DOI：
10.3389/fpls.2018.01909
复制
发表时间：
2018-12-21
影响因子：
5.6
通讯作者：
Li, Shaoqing
中科院分区：
文献类型：
--
作者：
Jin, Jie;Long, Weixiong;Li, Shaoqing
种子活力是水稻种子品质的重要特征，在目前的水稻直播生产体系中，种子活力是促进水稻从土壤中迅速发芽、发育成强苗的重要因素。但以往对种子活力的研究主要集中在品种上，对野生稻的研究较少。利用重测序技术对152份野生稻回交自交系进行基因分型，分析了正常和人工老化处理下种子活力相关性状的qtl。共检测到36个qtl，其中萌发势（GP） 8个，发芽率（GR） 10个，苗长（SL） 9个，根长（RL） 9个。其中，鉴定出14个新的qtl。其中，6个qtl与发芽有关，8个qtl与老化胁迫下幼苗生长有关。幼苗长度的主要qtl q9SL1.1、q6SL1.1和q3SL1.1落在同一位点，精细定位的间隔约为90 Kb。与萌发相关的主要qtl q9GR8.1和q9GP8.1被精细定位到约90 Kb的区间。本研究将为水稻育种计划中高种子活力水稻的选育及这些基因的进一步克隆提供依据。
Seed vigor is an important character of seed quality that promotes rice to germinate rapidly from soil and developing to a strong seedling, especially in the current rice direct-sowing production system. However, previous studies for seed vigor mainly concentrate in cultivars, and less reports involving in wild rice. In this study, 152 backcross inbred lines (BILs) derived from wild rice Oryza longistaminata were genotyped with resequencing technology, and QTLs for seed vigor related traits under normal and artificial aging treatment were analyzed. Totally, 36 QTLs were detected, of which, eight for germination potential (GP), 10 for germination rate (GR), 9 for seedling length (SL), and 9 for root length (RL). Among these, 14 novel QTLs were identified from O. longistaminata. Of which, six QTLs were related to germination, and eight related to seedling growth under aging stress. What's more, the major QTLs q9SL1.1, q6SL1.1, and q3SL1.1 for seedling length were fallen in the same locus and fine-mapped an interval about 90 Kb. The major QTLs q9GR8.1 and q9GP8.1 related with germination were fine-mapped to an interval about 90 Kb. This work will provide us basis for breeding of high seed vigor rice in rice breeding programs and further cloning of these genes.