Novel heparin/alginate gel combined with basic fibroblast growth factor promotes nerve regeneration in rat sciatic nerve
Novel heparin/alginate gel combined with basic fibroblast growth factor promotes nerve regeneration in rat sciatic nerve
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DOI：
10.1002/jbm.a.30194
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发表时间：
2004-12-15
影响因子：
4.9
通讯作者：
Ide, C
中科院分区：
文献类型：
--
作者：
Ohta, M;Suzuki, Y;Ide, C
我们已经开发了一种海藻酸盐凝胶交联共价键再生真皮，神经和骨骼。最近，设计了一种由肝素和海藻酸盐与乙二胺共价交联而成的新型基质（H/ a凝胶）。可稳定释放具有生物活性的碱性成纤维细胞生长因子（bFGF） 1个月，是肝素结合生长因子之一。在本报告中，我们研究了这种新型H/A凝胶对大鼠坐骨神经再生的影响。在本研究中，H/A凝胶中添加bFGF的再生轴突在早期生长速度快于普通海藻酸盐凝胶中添加bFGF的再生轴突。髓鞘纤维在晚期有增大直径的趋势，向正常大小靠拢。植入的神经束显示出轻微的纤维化和良好的血管化。含bFGF的H/A凝胶血管化较普通海藻酸盐凝胶发达。这些结果表明，含bFGF的H/A凝胶不仅可以作为一种高效的细胞支架，而且可以作为bFGF用于周围神经再生的稳定基质。(C) 2004 Wiley期刊有限公司
We have developed an alginate gel crosslinked with covalent bonds for regeneration of dermis, nerve, and bone. Recently, a novel matrix (H/A gel) which consists of heparin and alginate covalently crosslinked with ethylenediamine, was designed. It can stabilize and release biologically active basic fibroblast growth factor (bFGF) for 1 month, which is one of the heparin-binding growth factors. In the present report, we examined the effect of this novel H/A gel on nerve regeneration in the rat sciatic nerve. In this study, regenerated axons in H/A gel with bFGF grew faster than in ordinary alginate gel with bFGF in the early stage. Myelinated fibers showed a tendency to increase in diameter to ward the normal size in the later stage. Nerve bundles in the implantation exhibited minimal fibrosis and good vascularization. H/A gel with bFGF exhibited better-developed vascularization than ordinary alginate gel with bFGF. These findings suggested that H/A gel with bFGF could serve not only as an efficient cellular scaffold, but also as a stabilizing matrix for bFGF for peripheral nerve regeneration. (C) 2004 Wiley Periodicals, Inc.