Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection
Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection
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DOI：
10.1016/s0014-5793(98)01523-3
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发表时间：
1998-12-11
期刊：
影响因子：
3.5
通讯作者：
Reiländer, H
中科院分区：
文献类型：
--
作者：
Marheineke, K;Grünewald, S;Reiländer, H
通过高效液相色谱和气液色谱分析了来自草地贪夜蛾 (Sf9) 和粉纹夜蛾 (Tn) 的两种不同昆虫细胞系的脂质组成，这两种细胞系是建立的用于感染重组杆状病毒的细胞系。磷脂主要为磷脂酰胆碱和磷脂酰乙醇胺，主要单不饱和脂肪酸为油酸和棕榈油酸，主要饱和脂肪酸为硬脂酸。胆固醇与磷脂的比率被证明低于哺乳动物细胞系。重组杆状病毒苜蓿银纹夜蛾的感染导致昆虫细胞中磷脂酰胆碱的水平增加。杆状病毒/昆虫细胞系统已成为异源蛋白质生产的流行系统。这两种细胞系中产生的膜蛋白的功能变化可能与其细胞膜的不同脂质谱相关。 (C) 1998 年欧洲生化学会联合会。
The lipid composition of two different insect cell lines from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) which are established cell lines for infection with recombinant baculovirus was analyzed by high-performance liquid chromatography and gas-liquid chromatography. The major phospholipids found were phosphatidylcholine and phosphatidylethanolamine, the major mono-unsaturated fatty acids were oleic acid and palmitoleic acid, the major saturated fatty acid was stearic acid. The cholesterol to phospholipid ratio was demonstrated to be lower than in mammalian cell lines. Infection with a recombinant baculovirus Autographa californica resulted in increased levels of phosphatidylcholine in the insect cells. The baculovirus/insect cell system has become a popular system for heterologous protein production. Functional changes of membrane proteins produced in these two cell lines might be correlated to a different lipid profile of their cellular membranes. (C) 1998 Federation of European Biochemical Societies.