The anaphase promoting complex/cyclosome is recruited to centromeres by the spindle assembly checkpoint
The anaphase promoting complex/cyclosome is recruited to centromeres by the spindle assembly checkpoint
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DOI：
10.1038/ncb1167
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发表时间：
2004-09-01
影响因子：
21.3
通讯作者：
Pines, J
中科院分区：
文献类型：
--
作者：
Acquaviva, C;Herzog, F;Pines, J
后期促进复合物/细胞周期体（APC/C）对控制细胞分裂至关重要（综述见参考文献1）。它是一种多亚基泛素连接酶，在有丝分裂过程中的特定点，靶向特定蛋白质进行蛋白酶体降解。APC/C本身由纺锤体或动粒检查点调节，其在通过防止姐妹染色单体分离直到所有染色体在有丝分裂纺锤体上正确对齐来维持基因组稳定性方面具有重要作用。纺锤体检查点通过失活APC/C的重要共激活因子Cdc 20来调节APC/C。也有证据表明，纺锤体检查点组件和Cdc 20的有丝分裂装置的空间调节，特别是他们被招募到不正确连接的动粒。在这里，我们发现APC/C本身与纺锤体检查点的组件共同定位到不正确连接的动粒。事实上，我们提供的证据表明，纺锤体检查点机械所需的招募APC/C的着丝粒。我们的数据表明，APC/C可以直接由纺锤体检查点调节。
The anaphase promoting complex/cyclosome (APC/C) is crucial to the control of cell division (for a review, see ref. 1). It is a multi-subunit ubiquitin ligase that, at defined points during mitosis, targets specific proteins for proteasomal degradation. The APC/C is itself regulated by the spindle or kinetochore checkpoint, which has an important role in maintaining genomic stability by preventing sister chromatid separation until all chromosomes are correctly aligned on the mitotic spindle. The spindle checkpoint regulates the APC/C by inactivating Cdc20, an important co-activator of the APC/C. There is also evidence to indicate that the spindle checkpoint components and Cdc20 are spatially regulated by the mitotic apparatus, in particular they are recruited to improperly attached kinetochores. Here, we show that the APC/C itself co-localizes with components of the spindle checkpoint to improperly attached kinetochores. Indeed, we provide evidence that the spindle checkpoint machinery is required to recruit the APC/C to kinetochores. Our data indicate that the APC/C could be regulated directly by the spindle checkpoint.