Proteoglycan components of the intervertebral disc and cartilage endplate: an immunolocalization study of animal and human tissues.
Proteoglycan components of the intervertebral disc and cartilage endplate: an immunolocalization study of animal and human tissues.
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椎间盘和软骨终板的蛋白多糖成分：动物和人体组织的免疫定位研究。
DOI：
10.1007/bf00160052
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Eisenstein,SM
中科院分区：
文献类型：
--
作者：
Roberts,S;Caterson,B;Evans,H;Eisenstein,SM
单抗已被用来研究蛋白多糖分子的各种成分在椎间盘和软骨终板中的存在和分布。在人类和其他哺乳动物的组织中，已经研究了连接蛋白、透明质酸结合区、硫酸角蛋白和软骨素4-和6-硫酸盐。碳水化合物和蛋白质表位的暴露分别通过软骨素酶和胰酶的预处理而增加。免疫反应强度因部位不同而不同，髓核的免疫反应强度大于纤维环，而软骨终板的免疫反应最弱。此外，细胞周域的染色增加，特别是在成体组织中。异位钙化区域表现出非常不同的免疫反应，这取决于存在的钙盐的类型。与周围未钙化的基质相比，钙羟基磷灰石沉积物对8A4(链接蛋白)的染色更强，而焦磷酸钙沉积物对3B3(-)、7D4(-)和3D5的染色更强。硫酸软骨素异构体表位3B3(-)和7D4(-)的染色表明，在变性的人体组织中，硫酸软骨素的异构体表位比非变性的更明显。这表明，这些表位的表达可能是椎间盘和软骨终板疾病和后续修复过程的指标，类似于关节软骨退变。
Monoclonal antibodies have been used to study the presence and distribution of various components of the proteoglycan molecule in the intervertebral disc and cartilage endplate. Link protein, hyaluronic acid binding region, keratan sulphate and chondroitin 4- and 6-sulphate have been investigated in tissues from humans and other mammals. Exposure of the carbohydrate and protein epitopes was enhanced by chondroitinase and trypsin pretreatment respectively. The degree of immunoreactivity varied with location, being greater in the nucleus pulposus than the annulus fibrosus with least reactivity in the cartilage endplate. In addition, there was increased staining in the pericellular domains, particularly in adult tissues. Areas of ectopic calcification exhibited very different immunoreactivity, depending on the type of calcium salt present. Calcium hydroxyapatite deposits showed greater staining for 8A4 (link protein), while calcium pyrophosphate deposits demonstrated greater staining for 3B3(-), 7D4(-) and 3D5 than the surrounding non-calcified matrix. Staining for chondroitin sulphate isomer epitopes 3B3(-) and 7D4(-), indicative of modified chondroitin sulphate chains, was greater in human tissues of degenerate than non-degenerate appearance. This suggests that expression of these epitopes may be an indicator of disease and subsequent reparative procedures in intervertebral disc and cartilage endplate, similar to that seen in articular cartilage degeneration.