Modulating effects of a functional food containing Dendrobium officinale on immune response and gut microbiota in mice treated with cyclophosphamide
Modulating effects of a functional food containing Dendrobium officinale on immune response and gut microbiota in mice treated with cyclophosphamide
复制标题
含有铁皮石斛的功能性食品对环磷酰胺治疗小鼠的免疫反应和肠道微生物群的调节作用
DOI：
10.1016/j.jff.2022.105102
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发表时间：
2022-05-13
影响因子：
5.6
通讯作者：
Chen, Su-Hong
中科院分区：
文献类型：
--
作者：
Dong, Ying-Jie;Lin, Min-Qiu;Chen, Su-Hong
国家草药饮料是经国家市场监督管理总局批准的免疫增强功能食品。本研究研究了NHD对环磷酰胺（Cyclophosphamide， CTX）诱导的小鼠免疫调节和肠道菌群失调的影响，以阐明免疫抑制的衰减是否与NHD对肠道菌群的调节有关。我们的研究表明，CTX诱导免疫抑制小鼠免疫器官损伤，血清免疫球蛋白IgG、IgM和补体C3水平降低，骨髓造血功能中断，伴有肝毒性改变和结肠长度缩短。然而，所有这些变化都在不同程度上被NHD干预逆转。小鼠的延迟型超敏反应（DTH）和血清溶血素也表明NHD可以有效调节机体的细胞和体液免疫。此外，值得注意的是，NHD还可以改善肠道病理，提高肠道紧密连接蛋白Occludin和Claudin-1的表达，恢复肠道屏障功能。此外，α -多样性和β -多样性分析表明，NHD对肠道微生物群具有积极的调节作用。在门水平上，NHD可以调节拟杆菌门、厚壁菌门和放线菌门三个主要门的丰度。在物种水平上，NHD干预后，有益属unculred_bacterum_g_ norank_f_ Muribaculaceae的丰度增加，有害属unclassified_f_lachnospiraceae的丰度减少。这些结果为NHD通过恢复肠道屏障功能和调节肠道微生物群紊乱来调节环磷酰胺诱导的免疫功能低下小鼠的免疫反应提供了证据。
National Herbal Drink (NHD) is a functional food approved by the State Administration for Market Regulation for immune enhancement. In this study, the effects of NHD on Cyclophosphamide (CTX)-induced immune regulation and gut microbiota dysregulation in mice were investigated to elucidate whether the attenuation of immunosuppression was related to the regulation of the gut microbiota by NHD. Our study showed that CTX induced immune organ damage in immunosuppressed mice, with reduced levels of serum immunoglobulins IgG, IgM, and complement C3 and disruption of bone marrow hematopoiesis, accompanied by changes in liver toxicity and shortened colonic length. However, all these changes were reversed to varying degrees by NHD intervention. Delayed-type hypersensitivity (DTH) and serum hemolysins in mice also indicated that NHD could effectively regulate cellular and humoral immunity in the body. In addition, it is noteworthy that NHD can also improve intestinal pathology, elevate the expression of intestinal tight junction proteins Occludin and Claudin-1 to restore intestinal barrier function. Furthermore, alpha-diversity and beta-diversity analysis showed that NHD has a positive regulatory effect on the gut microbiota. At the phylum level, NHD could regulate the abundance of three major phyla, namely, Bacteroidetes, Firmicutes, and Actinobacteriota. At the species level, the abundance of the beneficial genus uncultured_bacterium_g__norank_f__ Muribaculaceae increased and the abundance of the harmful genus unclassified_f__Lachnospiraceae decreased after the NHD intervention. These results provide evidence that NHD modulates the immune response in cyclophosphamide-induced immunocompromised mice by restoring intestinal barrier function and regulating gut microbiota disorders.