Intranasal immunization with human papillomavirus type 16 capsomeres in the presence of non-toxic cholera toxin-based adjuvants elicits increased vaginal immunoglobulin levels
Intranasal immunization with human papillomavirus type 16 capsomeres in the presence of non-toxic cholera toxin-based adjuvants elicits increased vaginal immunoglobulin levels
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DOI：
10.1016/j.vaccine.2005.11.060
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发表时间：
2006-03-20
期刊：
影响因子：
5.5
通讯作者：
Gissmann, L
中科院分区：
文献类型：
--
作者：
Dell, K;Koesters, R;Gissmann, L
针对人乳头瘤病毒（HPV）的预防性免疫的目的优先在于产生针对病毒衣壳蛋白的抗体。无DNA的病毒样颗粒或其五聚体亚基，壳粒代表合适的抗原。在这里，我们研究了是否可以通过共同应用无毒的霍乱毒素佐剂CTA 1-D2 D1或CTB增强C57 B16小鼠血清和阴道冲洗液中的衣壳蛋白鼻内免疫诱导的抗HPV 16 L1特异性抗体和U特异性CTL。我们发现CTA 1-D2 D1以剂量依赖性方式升高L1特异性血清IgG抗体，CTA 1-D2 D1和CTB均显著增加阴道腔中L1特异性伊加抗体水平。CTA 1-D2 D I和CTB增强了L1特异性CTL应答。(c)2005爱思唯尔有限公司保留所有权利。
Prophylactic immunization against human papillomaviruses (HPVs) aims preferentially at the generation of antibodies, which are directed against the virus capsid proteins. DNA-free virus-like particles or their pentameric subunits, the capsomeres represent suitable antigens. Here we investigated if anti-HPV16 L1 specific antibodies and U-specific CTL induced by intranasal immunization with capsomeres in sera and vaginal washings of C57B16 mice can be enhanced by co-application of the non-toxic cholera toxin adjuvants CTA1-D2D1 or CTB. We found that CTA1-D2D1 elevated L1-specific serum IgG antibodies in a dose-dependent manner and both CTA1-D2D1 and CTB significantly increased L1-specific IgA antibody levels in the vaginal lumen. Furthermore, CTA1-D2D I and CTB enhanced L1-specific CTL responses. (c) 2005 Elsevier Ltd. All rights reserved.