Reduced CD27 Expression on Antigen-Specific CD4+ T Cells Correlates with Persistent Active Tuberculosis
Reduced CD27 Expression on Antigen-Specific CD4+ T Cells Correlates with Persistent Active Tuberculosis
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DOI：
10.1007/s10875-010-9418-1
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发表时间：
2010-07-01
影响因子：
9.1
通讯作者：
Cheng, Xiaoxing
中科院分区：
文献类型：
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作者：
Jiang, Jing;Wang, Xianyuan;Cheng, Xiaoxing
CD27是肿瘤坏死因子受体家族的成员，在T细胞免疫生成中起重要作用。本研究探讨分枝杆菌抗原特异性CD4(+)T细胞表面CD27的表达与肺结核的关系。根据CD154的表达鉴定分枝杆菌抗原特异性的CD4(+)T细胞，并用流式细胞术分析抗原特异性的CD27的表达。与结核菌素阳性的对照组相比，细菌培养阳性的肺结核患者抗原特异性的CD27的表达显著降低。持续活动性肺结核患者CD27(+)抗原特异性CD4T细胞百分率明显低于培养阳性的初治肺结核患者(P=0.008)和健康对照组(P=0.005)。Logistic回归分析显示，低比例的CD27(+)抗原特异性CD4T细胞与持续活动性肺结核有显著相关性(P=0.002，优势比=19.6)。结论CD27(+)抗原特异性CD4T细胞频率可作为持续活动性肺结核的免疫学标志物。
CD27, a member of the tumor necrosis factor receptor family, has important role in generation of T cell immunity. In this study, association of CD27 expression on mycobacterial antigen-specific CD4(+) T cells with pulmonary tuberculosis (TB) was investigated.Mycobacterial antigen-specific CD4(+) T cells were identified based on CD154 expression and CD27 expression on antigen-specific CD4 T cells was analyzed by flow cytometry.Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells. The persistent active TB patients had much lower percentages of CD27(+) antigen-specific CD4 T cells than culture-positive new TB patients (P = 0.008) and healthy controls (P = 0.005). Logistic regression analysis on frequencies of CD27-expressing antigen-specific CD4 T cells and TB patients' clinical characteristics indicated that low percentage of CD27(+) antigen-specific CD4 T cells correlated significantly with persistent active tuberculosis (P = 0.002, odds ratio = 19.6).It is concluded that frequency of CD27(+) antigen-specific CD4 T cells could be used as an immunological marker for persistent active TB.