Acylated flavonol monorhamnosides, α-glucosidase inhibitors, from Machilus philippinensis
Acylated flavonol monorhamnosides, α-glucosidase inhibitors, from Machilus philippinensis
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DOI：
10.1016/j.phytochem.2008.06.006
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发表时间：
2008-09-01
期刊：
影响因子：
3.8
通讯作者：
Chen, Chien-Kuang
中科院分区：
文献类型：
--
作者：
Lee, Shoei-Sheng;Lin, Hsiao-Ching;Chen, Chien-Kuang
生物活性导向的菲律宾润楠叶提取物活性成分的分级分离。当对嗜热脂肪芽孢杆菌（Bacillus stearothermophilus）进行测试时，产生了两种活性化合物，山奈酚-3-O-α-L-吡喃鼠李糖苷3“，4”-二-E-对香豆酸酯（1）和3“-E，4”-Z-二-对香豆酸酯（2）。葡萄糖苷酶IV型。1和2的IC（50）值分别为6.10和1.00 μ M。进一步应用HPLC-SPE-NMR联用技术在线表征CH（2）Cl（2）-可溶性级分中存在的其他活性成分，鉴定出毛地黄黄酮（3）和另外7种3-O-（香豆酰基-吡喃鼠李糖基）-黄酮醇（4-10）。它们的结构主要通过（1）H NMR光谱分析确定。在所鉴定的化合物中，化合物2、4、5和7是迄今未知的天然产物。(C)2008爱思唯尔有限公司保留所有权利。
Bioassay-guided fractionation and isolation of the active constituents from the leaf extract of Machilus philippinense Merr. yielded two active compounds, kaempferol-3-O-alpha-L-rhamnopyranoside 3 '',4 ''-di-E-p-coumaroic acid ester (1) and 3 ''-E,4 ''-Z-di-p-coumaroic acid ester (2) when tested against a Bacillus stearothermophilus, a or.-glucosidase type IV. The IC(50) values of 1 and 2 were 6.10 and 1.00 mu M, respectively. Further application of the HPLC-SPE-NMR hyphenated technique in the on-line characterization of other active ingredients present in the CH(2)Cl(2) - Soluble fraction led to identification of luteolin (3) and seven additional 3-O-(coumaroyl-rhamnopyranosyl)-flavonols (4-10). Their structures were determined mainly by (1)H NMR spectroscopic analyses. Among the compounds identified, compounds 2, 4, 5, and 7 were hitherto unknown natural products. (C) 2008 Elsevier Ltd. All rights reserved.