The interferon system of teleost fish
The interferon system of teleost fish
复制标题
DOI：
10.1016/j.fsi.2005.01.010
复制
发表时间：
2006-02-01
影响因子：
4.7
通讯作者：
Robertsen, B
中科院分区：
文献类型：
--
作者：
Robertsen, B
干扰素（IFN）是一种分泌性蛋白质，可诱导脊椎动物细胞进入抗病毒状态。在哺乳动物中，IFN的三个家族（I型IFN、II型IFN和IFN-λ）可以根据基因结构、蛋白质结构和功能特性来区分。I型IFN，包括IFN-α和IFN-β，由内含子缺失基因编码，在抵抗病毒的第一道防线中起主要作用。人IFN-λ具有与I型IFN相似的生物学特性，但由含有内含子的基因编码。II型IFN与IFN-γ相同，IFN-γ由T辅助I细胞响应于有丝分裂原和抗原而产生，并且在适应性细胞介导的免疫中具有关键作用。IFN，其显示类似于哺乳动物I型IFN的结构和功能特性，最近已从大西洋鲑鱼、癍点叉尾鱼、河豚和斑马鱼中克隆。硬骨鱼似乎至少有两个I型干扰素基因。系统发育序列分析表明，鱼类I型IFN形成一个组，与鸟类I型IFN和哺乳动物IFN-α，-β和-λ组分开。有趣的是，鱼IFN具有与IFN-λ相同的外显子/内含子结构，但与IFN-α显示出最大的序列相似性。最近，IFN-γ基因也被从几种鱼类中克隆出来，并显示出具有与哺乳动物IFN-γ基因相同的外显子/内含子结构。哺乳动物I型IFN的抗病毒作用是通过与IFN-α/β受体结合而发挥的，该受体通过JAK-STAT信号转导途径触发信号转导，导致Mx和其他抗病毒蛋白的表达。推测的干扰素受体基因已被确定在河豚。几种干扰素调节因子和JAK-STAT途径的成员也已在各种鱼类中鉴定。此外，Mx和其他几个干扰素刺激基因已在鱼类中克隆和研究。此外，来自大西洋鲑鱼和日本比目鱼的Mx蛋白的抗病毒活性最近已被证明。(c)2005爱思唯尔有限公司保留所有权利。
Interferons (IFNs) are secreted proteins, which induce vertebrate cells into an antiviral state. In mammals, three families of IFNs (type I IFN, type II IFN and IFN-lambda) can be distinguished on the basis of gene structure, protein structure and functional properties. Type I IFNs, which include IFN-alpha and IFN-beta are encoded by intron lacking genes and have a major role in the first line of defense against viruses. The human IFN-lambda s have similar biological properties as type I IFNs, but are encoded by intron containing genes. Type II IFN is identical to IFN-gamma, which is produced by T helper I cells in response to mitogens and antigens and has a key role in adaptive cell mediated immunity. IFNs, which show structural and functional properties similar to mammalian type I IFNs, have recently been cloned from Atlantic salmon, channel catfish, pufferfish, and zebrafish. Teleost fish appear to have at least two type I IFN genes. Phylogenetic sequence analysis shows that the fish type I IFNs form a group separated from the avian type I IFNs and the mammalian IFN-alpha, -beta and -lambda groups. Interestingly, the fish IFNs possess the same exon/intron structure as the IFN-lambda s, but show most sequence similarity to IFN-alpha. Recently, IFN-gamma genes have also been cloned from several fish species and shown to have the same exon/intron structure as mammalian IFN-gamma genes.The antiviral effect of mammalian type I IFN is exerted through binding to the IFN-alpha/beta- receptor, which triggers signal transduction through the JAK-STAT signal transduction pathway resulting in expression of Mx and other antiviral proteins. Putative IFN receptor genes have been identified in pufferfish. Several interferon regulatory factors and members of the JAK-STAT pathway have also been identified in various fish species. Moreover, Mx and several other interferon stimulated genes have been cloned and studied in fish. Furthermore, antiviral activity of Mx protein from Atlantic salmon and Japanese flounder has recently been demonstrated. (c) 2005 Elsevier Ltd. All rights reserved.