ROLE OF EXTRAVASCULAR GAS-BUBBLES IN SPINAL-CORD INJURY-INDUCED BY DECOMPRESSION-SICKNESS IN THE RAT
ROLE OF EXTRAVASCULAR GAS-BUBBLES IN SPINAL-CORD INJURY-INDUCED BY DECOMPRESSION-SICKNESS IN THE RAT
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DOI：
10.1006/exmp.1994.1022
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发表时间：
1994-08-01
影响因子：
3.6
通讯作者：
YIN, A
中科院分区：
文献类型：
--
作者：
MARZELLA, L;YIN, A
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We have evaluated the contribution of extravascular gas bubbles to spinal cord injury in decompression sickness. For this purpose, a model of decompression sickness was developed by subjecting rats to simulated dives using compressed air. Various diving profiles were tested and the presence of spinal cord injury was demonstrated by electrophysiologic measurements. To evaluate the space occupying lesions induced by gas bubbles in the white matter, the spinal cord was fixed by perfusion with 10% buffered formalin. Tissue blocks from cervical, thoracic, and lumbar spinal cords were embedded in paraffin. Tissue sections were double stained with luxol fast blue and hematoxylin and eosin. The space occupying lesions were quantified with a digitizer tablet. The fractional area of the lesions was 0.009% in controls and 0.026% in rats subjected to diving. We conclude that the volume of extravascular free gas present in the cord of rats with spinal decompression sickness is small and that artifacts of tissue preparation contribute to the volume estimate. As far as can be judged from the results in this animal model, the contribution of extravascular gas bubbles to spinal cord decompression injury is minor. (C) 1994 Academic Press, Inc.