Patterning of the heart field in the chick
Patterning of the heart field in the chick
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DOI：
10.1016/j.ydbio.2008.04.014
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发表时间：
2008-07-15
影响因子：
2.7
通讯作者：
Kirby, Margaret L.
中科院分区：
文献类型：
--
作者：
Abu-Issa, Radwan;Kirby, Margaret L.
在人类发育过程中，根据组织切片的假设，心源性中胚层与心包腔一起旋转180度。这也被认为是在小鼠发育过程中的情况，其中基因表达数据表明，右心室和流出道的祖细胞相对于心房和左心室的祖细胞颠倒了位置。然而，这两种情况下的倒置都是推断的，从来没有直接显示出来。我们在鸡胚胎中使用了3D重建和细胞示踪，以显示心源性中胚层的组织方式，即后半部分细胞被合并到心脏流入(心房和左心室)中，而中间放置的细胞被合并到心脏流出(右室和流出道)中。这是因为心源性中胚层是倒置的。内翻伴随着前肠入口的运动，后者向尾部滚动形成前肠口袋。双侧颅心原野内侧折叠、腹侧折叠、融合。心脏环扎后，腹侧融合形成的缝隙将成为心环的较大曲率。心源性中胚层的尾部边缘在背侧与内曲率重合。根据这张新的命运图对选定的心源性中胚层区域进行物理消融，证实了这些结果，此外，还表明右和左心房来自右和左心野。倒置和新的命运图解释了几个无法解释的观察结果，并为鸟类和哺乳动物提供了一个关于心场和心管形成的统一概念。(C)2008 Elsevier Inc.保留所有权利。
In human development, it is postulated based on histological sections, that the cardiogenic mesoderm rotates 180 degrees with the pericardial cavity. This is also thought to be the case in mouse development where gene expression data suggests that the progenitors of the right ventricle and outflow tract invert their position with respect to the progenitors of the atria and left ventricle. However, the inversion in both cases is inferred and has never been shown directly. We have used 3D reconstructions and cell tracing in chick embryos to show that the cardiogenic mesoderm is organized such that the lateralmost cells are incorporated into the cardiac inflow (atria and left ventricle) while medially placed cells are incorporated into the cardiac outflow (right ventricle and outflow tract). This happens because the cardiogenic mesoderm is inverted. The inversion is concomitant with movement of the anterior intestinal portal which rolls caudally to form the foregut pocket. The bilateral cranial cardiogenic fields fold medially and ventrally and fuse. After heart looping the seam made by ventral fusion will become the greater curvature of the heart loop. The caudal border of the cardiogenic mesoderm which ends up dorsally coincides with the inner curvature. Physical ablation of selected areas of the cardiogenic mesoderm based on this new fate map confirmed these results and, in addition, showed that the right and left atria arise from the right and left heart fields. The inversion and the new fate map account for several unexplained observations and provide a unified concept of heart fields and heart tube formation for avians and mammals. (c) 2008 Elsevier Inc. All rights reserved.