Isolation and characterization of rat liver nuclear matrices containing high molecular weight deoxyribonucleic acid.
Isolation and characterization of rat liver nuclear matrices containing high molecular weight deoxyribonucleic acid.
复制标题
含有高分子量脱氧核糖核酸的大鼠肝核基质的分离和表征。
DOI：
10.1021/bi00520a028
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Buchholtz,LA
中科院分区：
文献类型：
--
作者：
Berezney,R;Buchholtz,LA
罗纳德Berezney* 和琳达A.用限制DNA降解的方法分离的大鼠肝细胞核基质含有总核DNA的主要部分。大多数DNA在> 100 S时在碱性蔗糖梯度上沉积，这表示估计的单链大小> 500个酶。这些富含DNA的基质在十二烷基硫酸钠-丙烯酰胺凝胶上回收总核蛋白和总多肽组成方面与先前分离的DNA耗尽基质几乎相同。薄切片电子显微镜显示的结构类似于DNA耗尽的矩阵，除了一个突出的网状DNA原纤维延伸整个矩阵内部。再生的体内标记
Ronald Berezney* and Linda A. Buchholtz abstract: Rat liver nuclear matricesisolated by a method which limits DNA degradation contain a major portion of the total nuclear DNA. A majority of the DNA sediments at> 100 S on alkaline sucrose gradients, which represents an estimated single strand size of> 500 kilobases. These DNA-rich matrices were virtually identical with previously isolated DNA-depleted matrices in recovery of total nuclear protein and overall polypeptide composition on sodium dodecyl sul-fate-acrylamide gels. Thin-sectioning electron microscopy revealed a structure similar to the DNA-depleted matrices with the addition of a prominent meshwork of DNA Fibrils extended throughout the matrix interior. In vivo labeling of regenerating