Biocompatible Gold Nanorods: One-Step Surface Functionalization, Highly Colloidal Stability, and Low Cytotoxicity
Biocompatible Gold Nanorods: One-Step Surface Functionalization, Highly Colloidal Stability, and Low Cytotoxicity
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DOI：
10.1021/acs.langmuir.5b00666
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发表时间：
2015-05-05
期刊：
影响因子：
3.9
通讯作者：
Gooding, J. Justin
中科院分区：
文献类型：
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作者：
Liu, Kang;Zheng, Yuanhui;Gooding, J. Justin
金纳米棒（aunr）与聚乙二醇（PEG）的偶联是降低其合成中使用的十六烷基三甲基溴化铵（CTAB）和银离子引起的细胞毒性的最有效方法之一。然而，典型的聚乙二醇化仅发生在aunr的尖端，产生部分修饰的aunr。为了解决这一问题，我们开发了一种新的、简单的、一步表面功能化方法，包括使用Tween 20来稳定AuNR，使用双(p-磺atophenyophenylphosphine （BSPP）来激活AuNR表面进行随后的聚乙二醇化，使用NaCl从AuNR上蚀刻银。这种方法允许从aunr中完全去除表面结合的CTAB和最活跃的表面银。制备的aunr对聚乙二醇化aunr的毒性远低于其他方法，当其浓度低于5 μ g/mL时无明显毒性。即使在80 μ g/mL的高浓度下，它们的细胞活力仍比尖端修饰的aunr高4倍。
The conjugation of gold nanorods (AuNRs) with polyethylene glycol (PEG) is one of the most effective ways to reduce their cytotoxicity arising from the cetyltrimethylammonium bromide (CTAB) and silver ions used in their synthesis. However, typical PEGylation occurs only at the tips of the AuNRs, producing partially modified AuNRs. To address this issue, we have developed a novel, facile, one-step surface functionalization method that involves the use of Tween 20 to stabilize AuNRs, bis(p-sulfonatophenyOphenylphosphine (BSPP) to activate the AuNR surface for the subsequent PEGylation, and NaCl to etch silver from the AuNRs. This method allows for the complete removal of the surface-bound CTAB and the most active surface silver from the AuNRs. The produced AuNRs showed far lower toxicity than other methods to PEGylate AuNRs, with no apparent toxicity when their concentration is lower than 5 mu g/mL. Even at a high concentration of 80 mu g/mL, their cell viability is still four times higher than that of the tip-modified AuNRs.