Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer
Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer
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DOI：
10.1006/jsre.2002.6356
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发表时间：
2002-06-15
影响因子：
2.2
通讯作者：
Tanaka, K
中科院分区：
文献类型：
--
作者：
Doi, K;Horiuchi, T;Tanaka, K
背景资料。肝缺血/再灌注(I/R)对肝转移的影响尚未得到充分研究。我们在大鼠结直肠癌模型中检测了肝I/R和肝转移，并对I/R后E-选择素(ELAM-1)的mRNA表达进行了定量。大鼠进行30或60min的70%部分肝缺血。再灌注60min后，将大鼠结肠腺癌细胞(RCN-H4)接种于脾内。3周后测定肝表面肿瘤结节数。用定量RT-PCR法检测缺血1h、3h、6h E-选择素mRNA的表达。肝脏I/R可促进RCN-H4的肝转移，并诱导E-选择素在阻断和开放肝叶中的表达。缺血60min后肿瘤结节数及E-选择素mRNA表达均高于30min。肝脏I/R，尤其是长时间的缺血，可诱导E-选择素的表达，促进结肠癌的肝转移。(C)2002年埃尔塞维尔科学公司(美国)。
Background. The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R.Materials and methods. Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR.Results. Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min.Conclusions. Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats. (C) 2002 Elsevier Science (USA).