ISOLATION OF LESS-THAN GLU-GLY-ARG-PHE-NH2 (ANTHO-RFAMIDE), A NEUROPEPTIDE FROM SEA-ANEMONES
ISOLATION OF LESS-THAN GLU-GLY-ARG-PHE-NH2 (ANTHO-RFAMIDE), A NEUROPEPTIDE FROM SEA-ANEMONES
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DOI：
10.1073/pnas.83.24.9817
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
GRAFF, D
中科院分区：
文献类型：
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作者：
GRIMMELIKHUIJZEN, CJP;GRAFF, D
已开发了用于含有羧基末端序列Arg-Phe-NH 2（RF酰胺）的肽的放射免疫测定法。利用这种放射免疫分析法，并应用阳离子交换色谱和HPLC，我们已经分离出的RF酰胺肽的乙酸提取物的海葵Anthopleura elegantissima。三种不同的方法确定了花侧耳RF酰胺肽（Antho-RF酰胺）的结构是
A radioimmunoassay has been developed for peptides containing the carboxyl-terminal sequence Arg-Phe-NH2 (RFamide). Using this radioimmunoassay and applying cation-exchange chromatography and HPLC, we have isolated an RFamide peptide from acetic acid extracts of the sea anemone Anthopleura elegantissima. Three different methods established that the structure of the Anthopleura RFamide peptide (Antho-RFamide) is