Bioactive Glass-Polycitrate Hybrid with Osteogenetic Ability Comparable to Autogenous Bone
Bioactive Glass-Polycitrate Hybrid with Osteogenetic Ability Comparable to Autogenous Bone
复制标题
生物活性玻璃-聚柠檬酸盐混合物，其成骨能力可与自体骨相媲美
DOI：
10.1166/jbn.2019.2709
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发表时间：
2019
影响因子：
2.9
通讯作者：
Zhang Zhongmin
中科院分区：
文献类型：
--
作者：
Zhao Huiyu;Ren Huihui;Zhang Jie;Wang Liang;Li Ailing;Tang Jiajun;Yan Bo;Zhou Wen;Lin Xuezhi;Ao Xiang;Chu Jun;Qiu Dong;Zhang Zhongmin
近年来人工骨移植取得了显著进展。然而，它们都没有接近自体骨的成骨能力。因此，提高人工骨的成骨能力是该领域最突出和最具挑战性的课题。此外，血管生成可以为细胞的生存提供稳定的环境和营养，对骨移植的成功也起着至关重要的作用。在本研究中，我们将柠檬酸盐聚合物和生物活性玻璃结合在一起作为分子水平的杂化物（PEC-GS/BG），期望获得与自体骨相当的成骨能力和血管生成能力来修复骨缺损。通过建立SD大鼠股骨髁临界缺损模型，对其进行体内外实验研究。在体内，术后12周，自体骨组骨密度（BMD）为（517 ± 21）mg/cm 3，与PEC-GS/BG组（509 ± 21）mg/cm 3相比，差异无统计学意义（p > 005）。测得同龄正常男性股骨髁骨密度为557 ± 16 mg/cm ~ 3，仅略高于上述数据，表明缺损几乎完全修复。PEC-GS/BG能刺激机体释放血管内皮生长因子（VEGF），促进血管新生。PEC-GS/BG的成骨能力接近自体骨，但血管生成能力更强。从蛋白质和细胞水平来看，PEC-GS/BG促进了MC 3 T3 E-1细胞的分化和矿化。因此，PEC-GS/BG在修复骨缺损方面的成骨性能与自体骨几乎相同。考虑到骨移植的高需求和自体骨供应的困难，本研究开发的PEC-GS/BG杂化物可能为骨修复开辟新的视野。
Significant progress in artificial bone grafts has been achieved in recent years. However, none of them has osteogenic ability that is close to autogenous bones. Hence, improving osteogenic ability of artificial bone is the most prominent and challenging task in this field. In addition, angiogenesis could provide a stable environment and nutrients for survival of the cells and also plays a crucial role to the success of bone transplantation. In the present study, we combined citrate polymer and bioactive glass together as hybrids at the molecular level (PEC-GS/BG), with the expectation of acquiring osteogenic ability and angiogenic ability to repair bone defect that could comparable to autogenous bones. In vitro and in vivo experiment on the femoral condyle critical defects model of Sprague-Dawley rats were conducted for a complete evaluation. In vivo, the bone mineral density (BMD) in defects was no significant difference between autogenous bone groups (517 ± 21 mg/cm3) and PEC-GS/BG groups (509 ± 21 mg/cm3) (p > 0 05) at 12 weeks post-surgery. The BMD of the femoral condyle in normal males at the same age was measured to be 557 ± 16 mg/cm3, only slightly higher than the above date, indicating a nearly complete repair of the defects. It was also found that PEC-GS/BG promoted angiogenesis due to it stimulated organism to release vascular endothecial growth factor (VEGF). PEC-GS/BG also showed great osteogenic ability that was close to autogeneous bones, but much better angiogenic ability. What's more, from both protein and cell levels, PEC-GS/BG accelerated differentiation and mineralization of MC3T3E-1 cells. Consequently, osteogenetic performance of PEC-GS/BG was almost same to autogenous bones in repairing bone defects. Considering the high demand in bone grafts and all the difficulties in autogeneous bone supply, the PEC-GS/BG hybrids developed in this study may open a new horizon for bone repair.