Development and maturation of the pediatric human vocal fold lamina propria
Development and maturation of the pediatric human vocal fold lamina propria
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DOI：
10.1097/01.mlg.0000150685.54893.e9
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发表时间：
2005-01-01
期刊：
影响因子：
2.6
通讯作者：
Prasad, V
中科院分区：
文献类型：
--
作者：
Hartnick, CJ;Rehbar, R;Prasad, V
Objective.目的：鉴定人声带固有层成熟的特征性模式。随着它发展成为一个成熟的结构。方法.采用苏木精-伊红、三色、pH 2.5阿辛蓝、Weigert网状染色和米勒弹性蛋白染色对34例0 - 18岁存档喉的真实声带切片进行组织学评价。地点三级儿童医院病理科。结果：出生后及出生后不久，在整个固有层中存在相对多细胞的单层细胞。到2月龄时，有分化成具有不同细胞群体密度的双层结构的第一迹象。在11个月和5岁之间，可以看到两种不同的模式：1）这种双层结构和2）固有层，其中存在第三个紧邻声带肌肉的细胞减少区域（该区域类似于表面上皮深处发现的浅表细胞减少区域）。到7岁时，根据细胞群的密度差异，所有标本都表现出中层和深层之间的这种过渡。一层固有层。由差别纤维组成（弹性蛋白和胶原纤维）定义的结构直到13岁才出现，然后在整个青春期出现。结论：使用经典的成人模型的纤维成分和密度来区分人类声带固有层的分层结构可能不足以允许一个完整的描述的过程中的成熟和发展。相反，早在产后2个月就可以看到不同的细胞密度区域，细胞分布模型可以更好地描述固有层的发育。塑造固有层形成的细胞信号传导过程似乎产生差异细胞密度的分层群体，其随后又产生差异纤维组合物。因此，可以通过评估这些不同细胞群的成熟来跟踪早期发育。未来的研究需要量化这些细胞分布模式，研究触发这种成熟的细胞信号传导过程，并将这些发现与机械建模相关联。
Objective. To identify characteristic patterns of maturation of the human vocal fold lamina propria. as it develops into a mature structure. Methods. Histologic evaluation of sectioned true vocal folds from 34 archived larynges ages 0 to 18 years using hematoxylineosin, trichrome, Alcian blue pH 2.5, Weigert reticular, and Miller's elastin stain. Location Pathology department at a tertiary care children's hospital. Results: At birth and shortly thereafter, there exists a relative hypercellular monolayer of cells throughout the lamina propria. By 2 months of age, there are the first signs of differentiation into a bilaminar structure of distinct cellular population densities. Between 11 months and 5 years, two distinct patterns are seen: 1) this bilaminar structure and 2) a lamina propria where there exists a third more hypocellular region immediately adjacent to the vocalis muscle (this region is similar to the superficial hypocellular region found just deep to the surface epithelium). By 7 years of age, all of the specimens exhibit this transition between the middle and the deeper layers according to differential density of cell populations. A lamina propria. structure defined by differential fiber composition (elastin and collagen fibers) is not present until 13 years of age and then is present throughout adolescence. Conclusions: Using the classic adult model of fiber composition and density to differentiate the layered structure of the lamina propria of the human vocal fold may not adequately allow for a thorough description of the process of maturation and development. Rather, distinct regions of cell density are seen as early as 2 months postpartum, and the model of cellular distribution may serve better to describe the lamina propria as it develops. Cell-signaling processes that shape the formation of the lamina propria appear to produce layered populations of differential cell density that in turn will later produce differential fiber compositions. Early development therefore can be followed by evaluating the maturation of these differing cell populations. Future studies are needed to quantify these cell distribution patterns, to study the cell signaling processes that trigger this maturation, and to correlate these findings with mechanical modeling.