Acetic acid activates PKD1L3-PKD2L1 channel-A candidate sour taste receptor
Acetic acid activates PKD1L3-PKD2L1 channel-A candidate sour taste receptor
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DOI：
10.1016/j.bbrc.2009.05.069
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发表时间：
2009-07-31
影响因子：
3.1
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
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作者：
Ishii, Sho;Misaka, Takumi;Abe, Keiko
多囊肾病（PKD）1 L3-PKD 2L 1通道是哺乳动物味觉受体细胞中表达的候选酸味受体。据报道，各种酸激活PKD通道后，删除的酸刺激，但很少有信息是这些通道的激活乙酸。这是很难分析PKD通道激活乙酸使用Ca 2+成像实验，因为这种酸诱导培养细胞的瞬时和非特异性的反应。在这里，我们开发了一种新的方法来评估PKD通道激活乙酸。非特异性反应仅在应用乙酸后的短时间内观察到，相比之下，即使在稍后的时间点也检测到由乙酸以及柠檬酸诱发的PKD通道激活。该方法揭示了PKD 1 L3-PKD 2L 1通道被乙酸激活是pH依赖性的，并且当环境pH为
The polycystic kidney disease (PKD) 1L3-PKD2L1 channel is a candidate sour taste receptor expressed in mammalian taste receptor cells. Various acids are reported to activate PKD channels after the removal of the acid stimuli, but little information is available on the activation of these channels by acetic acid. It was difficult to analyze the PKD channel activation by acetic acid using Ca2+ imaging experiments because this acid induces a transient and nonspecific response in cultured cells. Here, we developed a novel method to evaluate PKD channel activation by acetic acid. Nonspecific responses were observed only over a short period after the application of acetic acid, In contrast, PKD channel activation evoked by acetic acid as well as citric acid was detected even at a later time point. This method revealed that PKD1L3-PKD2L1 channel activation by acetic acid was pH-dependent and occurred when the ambient pH was