Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells.
Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells.
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DOI：
10.1002/cbic.201000414
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发表时间：
2011-01-03
期刊：
影响因子：
--
通讯作者：
Ting AY
中科院分区：
文献类型：
--
作者：
Jin X;Uttamapinant C;Ting AY
PRIME时间：我们报道了一种pH不敏感的蓝色荧光团，7-氨基香豆素，通过使用钯催化的Buchwald-Hartwig交叉偶联的合成。7-氨基香豆素可用于通过PRIME（酶介导的探针掺入）标记细胞表面和活细胞内的重组蛋白，与7-羟基香豆素不同，氨基香豆素可在酸性细胞器（如内体）中可视化。
PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium-catalyzed Buchwald–Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.