Neuronal degeneration and spinal cavitation following intraspinal injections of quisqualic acid in the rat.
Neuronal degeneration and spinal cavitation following intraspinal injections of quisqualic acid in the rat.
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DOI：
10.1089/neu.1993.10.445
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发表时间：
1993
影响因子：
4.2
通讯作者：
R. P. Yezierski;Margarita Santana;S. Park;Parley W. Madsen
中科院分区：
文献类型：
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作者：
R. P. Yezierski;Margarita Santana;S. Park;Parley W. Madsen
在大鼠脊髓中微量注射使君子酸，以评价这种兴奋性氨基酸激动剂对脊髓神经元的兴奋毒性作用。根据注射后7-49天的存活时间将动物分为四组。在下胸椎和上腰椎脊髓中注射0.3至2.0 μ L的8.3、83和125 mM使君子酸或生理盐水。在所有的生存时间评估单边注射使君子酸产生单边或双边细胞死亡和突出的炎症反应。在23/25只动物中还观察到脊髓腔。使君子酸注射部位或附近的脊髓节段含有深染、肥大的神经元轮廓和胶质细胞酸性因子染色增加。神经胶质细胞酸性因子的免疫染色在神经元变性的区域和脊髓腔的边缘区域特别强烈。本研究结果表明，脊髓内注射使君子酸可能是研究兴奋性氨基酸神经毒性机制以及神经元损伤后脊髓空洞发病机制的有效方法。
Microinjections of quisqualic acid were made in the spinal cord to evaluate the excitotoxic effects of this excitatory amino acid agonist on spinal neurons in the rat. Animals were divided into four groups based on post injection survival times of 7-49 days. Injections ranging from 0.3 to 2.0 microL of 8.3, 83, and 125 mM quisqualic acid or normal saline were made in the lower thoracic and upper lumbar spinal cord. At all survival times evaluated unilateral injections of quisqualic acid produced unilateral or bilateral cell death and a prominent inflammatory reaction. In 23/25 animals spinal cavities were also observed. Spinal cord segments at or near quisqualate injection sites contained darkly stained, hypertrophied neuronal profiles, and increased staining for glial fibrillary acidic factor. Immunostaining for glial fibrillary acidic factor was especially intense in areas of neuronal degeneration and in border areas of spinal cavities. The results of this study suggest that the intraspinal injection of quisqualic acid may be an effective method to study the mechanisms of excitatory amino acid neurotoxicity, and the pathogenesis of spinal cavitation following neuronal injury.