Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp ciceris
Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp ciceris
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DOI：
10.1006/pmpp.2003.0445
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发表时间：
2002-12-01
影响因子：
2.7
通讯作者：
Tena, M
中科院分区：
文献类型：
--
作者：
García-Limones, C;Hervás, A;Tena, M
探讨活性氧在尖孢镰刀菌引起的鹰嘴豆枯萎病中的作用。sp. ciceris的脂质过氧化程度（丙二醛形成）和二胺氧化酶（DAO）（一种质外体H2O2形成氧化酶）和几种抗氧化酶（即抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、愈创木酚依赖性过氧化物酶（GPX）和超氧化物歧化酶（SOD））的活性水平，在‘WR315’（抗病）和‘JG62’（感病）鹰嘴豆品种接种病原菌强毒小种5的根和茎中，用荧光定量法测定。此外，还采用凝胶电泳和活性染色法分析了根和茎中APX、CAT、GPX和SOD的含量，并采用Western blotting法测定了根中APX和SOD的蛋白水平。在根中，病原体的感染增加脂质过氧化和CAT和SOD活性，虽然这样的反应发生在不兼容的兼容的相互作用相比。APX，GPX和GR活性也增加，但仅在亲和互作。在茎中，病原菌侵染仅在亲和互作中增加了膜脂过氧化作用和APX、CAT、SOD和GPX活性，而在非亲和互作中增加了DAO活性。在一般情况下，酶谱图同意的活性水平测定，并没有发现任何差异，品种之间或感染和未感染的植物之间的同工酶模式。此外，Western印迹显示，在兼容的相互作用和SOD的兼容和不兼容的相互作用中的APX的根蛋白水平的增加。因此，茎中DAO活性的增强、根中脂质过氧化、CAT和SOD活性的提前升高与鹰嘴豆枯萎病抗性相关，而根和茎中后三个参数的诱导沿着APX、GR（仅根）和GPX（仅茎）活性的诱导与亲和互作的建立更相关。(C)2003爱思唯尔科技有限公司。保留所有权利。
To ascertain if active oxygen species play a role in fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris, the degree of lipid peroxidation (malondialdehyde formation) and the activity levels of diamine oxidase (DAO), an apoplastic H2O2-forming oxidase, and,several antioxidant enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol-dependent peroxidase (GPX) and superoxide dismutase (SOD), were determined spectrophotometrically in roots and stems of 'WR315' (resistant) and 'JG62' (susceptible) chickpea cultivars inoculated with the highly virulent race 5 of the pathogen. Moreover, APX, CAT, GPX and SOD were also analysed in roots and stems by gel electrophoresis and activity staining; and the protein levels of APX and SOD in roots were determined by Western blotting. In roots, infection by the pathogen increased lipid peroxidation and CAT and SOD activities, although such responses occurred earlier in the incompatible compared with the compatible interactions. APX, GPX and GR activities were also increased in infected roots; but only in the compatible interaction. In stems, infection by the pathogen increased lipid peroxidation and APX, CAT, SOD and GPX activities only in the compatible interaction, and DAO activity only in the incompatible one. In general, electrophoregrams agreed with the activity levels determined spectrophotometrically and did not reveal any differences in isoenzyme patterns between cultivars or between infected and noninfected plants. Further, Western blots revealed an increase in the root protein levels of APX in the compatible interaction and in those of SOD in both compatible and incompatible interactions. In conclusion, whereas enhanced DAO activity in stems, and earlier increases in lipid peroxidation and CAT and SOD activities in roots, can be associated with resistance to fusarium wilt in chickpea, the induction of the latter three parameters in roots and stems along with that of APX, GR (only in roots) and GPX (only in stems) activities are rather more associated with the establishment of the compatible interaction. (C) 2003 Elsevier Science Ltd. All rights reserved.