Guidelines for calibration of stimulus and recording parameters used in clinical electrophysiology of vision
Guidelines for calibration of stimulus and recording parameters used in clinical electrophysiology of vision
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DOI：
10.1023/a:1026244901657
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发表时间：
2003-09-01
影响因子：
1.4
通讯作者：
Robson, J
中科院分区：
文献类型：
--
作者：
Brigell, M;Bach, M;Robson, J
为了进行技术上合适的临床电生理程序，有必要对刺激和记录设备进行校准。已公布的视网膜电信号(ERG)[1]、眼电(EOG)[2]、视觉诱发电位(VEP)[3]、模式ERG(PERG)[4]和多焦ERG指南[5]规定了刺激和记录参数。然而，大多数商业仪器都不提供校准这些参数的手段。本文件的目的是为刺激和记录设备的正确校准提供指南。从临床和科学的角度来看，制定这样的指导方针的必要性是显而易见的。刺激和放大器特性对临床电生理学中常用的峰潜伏期和波幅测量有很大影响。许多关于临床电生理学的综述文章强调，需要根据年龄和性别为每个实验室建立规范，而不是依赖于已发表的规范。然而，如果不定期校准刺激和记录参数，则这些规范实际上可能会由于设备老化或无意中的设置改变而导致刺激或记录条件的改变而产生误导。本文件分为两个主要部分。第一个是关于视觉刺激的校准。它首先介绍有关光的物理及其测量的背景技术信息。紧随其后的是用于测量闪光刺激的发光强度和图案刺激的平均亮度、对比度和视角的协议。第二部分涉及电生理记录系统的校准。它首先描述生物电信号的特征及其测量。然后是测量电极阻抗和校准放大器的协议。尽管本文件是作为临床电生理测试指南编写的，但应注意的是，所描述的技术适用于任何依赖于准确测量亮度或电生理信号的研究。
In order to perform a technically adequate clinical electrophysiological procedure it is necessary to calibrate the stimulating and recording equipment. Published standards for the electroretinogram (ERG)[1], electro-oculogram (EOG)[2], visual evoked potential (VEP)[3], pattern ERG (PERG)[4] and guidelines for the multifocal ERG [5] specify stimulus and recording parameters. Yet most commercial instruments do not provide the means for calibration of these parameters. The goal of this document is to provide guidelines for proper calibration of stimulus and recording equipment. The need for such guidelines is clear on both clinical and scientific grounds. Stimulus and amplifier characteristics have substantial effects on the peak latency and amplitude measurements that are commonly used in clinical electrophysiology. Many review articles on clinical electrophysiology emphasize the need for establishing norms for each laboratory as a function of age and gender rather than relying on published norms. However, if stimulus and recording parameters are not calibrated periodically, then these norms may actually be misleading due to changes in stimulus or recording conditions induced by aging of equipment or inadvertent change in settings. This document is divided into two major sections. The first is concerned with calibration of the visual stimulus. It begins with background technical information on the physics of light and its measurement. This is followed by protocols for measurement of the luminous intensity of flash stimuli and the mean luminance, contrast, and visual angle of pattern stimuli. The second section is concerned with calibration of electrophysiological recording systems. It begins with a description of the characteristics of bioelectrical signals and their measurement. This is followed by protocols for measurement of electrode impedance and amplifier calibration. Although this document was prepared as guidelines for clinical electrophysiological testing, it should be noted that the techniques described are applicable to any studies that are dependent upon accurate measurement of luminance or electrophysiological signals.