Changes in collagen fiber content and hepatic stellate cell distribution in the liver of chick embryos and growing chickens
Changes in collagen fiber content and hepatic stellate cell distribution in the liver of chick embryos and growing chickens
复制标题
鸡胚胎和生长鸡肝脏中胶原纤维含量和肝星状细胞分布的变化
DOI：
10.1111/j.1740-0929.2011.00986.x
复制
发表时间：
2012
影响因子：
2
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Nishimura;et al
通过对肝星状细胞（HSCs）的分布和胶原蛋白含量的研究，揭示了胚胎和生长鸡肝脏性别依赖性变异的发生。分析了12 ~ 20日龄和1、4、8周龄的鸡胚。肝组织用氢氧化钠浸渍和冷冻干燥得到胶原纤维标本。采用双荧光免疫组化法检测血凝蛋白和血凝蛋白。在胚胎阶段，每肝重量和HSC面积的胶原纤维百分比没有性别依赖性变化。然而，在胚胎发育过程中，两性的胶原纤维含量都有所增加。另一方面，生长中的雄性造血干细胞面积明显增加，而雌性没有变化。重要的是，在8周时观察到肝脏中胶原纤维含量和HSC面积的性别差异。这些结果表明，鸡肝脏中胶原含量的变化发生在8周时，这表明一种性别依赖性激素可能对生长中的鸡肝脏中hsc的胶原生成起重要作用。
The content of collagen and the distribution of hepatic stellate cells (HSCs) were studied to elucidate the occurrence of sex‐dependent variations in the liver of developing embryos and growing chickens. Chick embryos from embryonic days (e) 12 to e20 and chicks at 1, 4 and 8 weeks were analyzed. Liver tissue was processed using NaOH maceration and freeze‐dried to obtain the collagen fiber specimens. HSCs were identified by double fluorescent immunohistochemistry for desmin and vimentin. There were no sex‐dependent variations in the percentage of collagen fiber per liver weight and HSC area during embryonic stages. However, the content of collagen fiber increased during embryonic development in both sexes. On the other hand, the area of HSCs significantly increased in growing males but did not show any change in females. Importantly, sex differences were observed in both collagen fiber content and HSC area in the liver at 8 weeks. These results indicate that the occurrence of collagen content variations takes place at 8 weeks in chicken liver, suggesting that a sex‐dependent hormone may play an important role on the collagen production of HSCs in the growing chicken liver.