Specific overexpression of OLFM4GW112/hGC-1 mRNA in colon, breast and lung cancer tissues detected using quantitative analysis
Specific overexpression of OLFM4GW112/hGC-1 mRNA in colon, breast and lung cancer tissues detected using quantitative analysis
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DOI：
10.1111/j.1349-7006.2006.00383.x
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发表时间：
2007-03-01
期刊：
影响因子：
5.7
通讯作者：
Watanabe, Naoki
中科院分区：
文献类型：
--
作者：
Koshida, Saori;Kobayashi, Daisuke;Watanabe, Naoki
近年来研究发现，嗅觉调节蛋白4（olfactomedin 4，OLFM 4（GW 112，/hGC-1））基因的过表达可抑制多种凋亡途径，促进癌细胞增殖，提示OLFM 4可能作为人类癌症的诊断标志物。因此，我们研究了癌症特异性OLFM 4过表达。OLFM 4 mRNA在结肠、乳腺和肺的癌组织中高度表达。在68%和50%的研究的结肠癌和乳腺癌组织中观察到OLFM 4 mRNA的阳性，定义为非癌性结肠癌和乳腺癌组织中的平均值± 2 SD。OLFM 4 mRNA在非癌肺组织中未检测到，但在62%的肺癌组织中明显表达。通过比较配对样本，观察到OLFM 4 mRNA的表达在90%、69%和85%的结肠癌、乳腺癌和肺癌组织中分别升高。OLFM 4 mRNA的表达甚至在每种癌症类型的早期阶段都被观察到。OLFM 4 mRNA的表达与抗凋亡分子Survivin的表达无相关性，提示OLFM 4 mRNA可独立用于肿瘤诊断。OLFM 4和生存素的组合导致更高的阳性率。因此，OLFM 4 mRNA可能是一个有用的工具，以支持癌症的诊断，无论临床阶段。
Overexpression of the olfactomedin 4 (OLFM4(GW112,/hGC-1)) gene was recently reported to inhibit various apoptotic pathways and promote proliferation of cancer cells, suggesting that OLFM4 might serve as a diagnostic marker for human cancers. Therefore, we examined cancer-specific OLFM4 overexpression. OLFM4 mRNA was highly expressed in cancerous tissues obtained from the colon, breast and lung. Positivity for OLFM4 mRNA, defined as the mean + 2 SD in non-cancerous colon and breast tissues, was observed in 68 and 50% of the studied colon and breast cancer tissues. OLFM4 mRNA expression was not detected in non-cancerous lung tissues but was evident in 62% of the lung cancer tissues. On comparing paired samples, the expression of OLFM4 mRNA was observed to be elevated in 90, 69 and 85% of colon, breast and lung cancer tissues, respectively. OLFM4 mRNA expression was observed even in the early stages of each cancer type. The expression of OLFM4 mRNA did not correlate with that of the antiapoptotic molecule survivin, indicating that it can be used independently in cancer diagnosis. Combining OLFM4 and survivin resulted in higher positivity. Thus, OLFM4 mRNA might be a useful tool to support the diagnosis of cancer, irrespective of the clinical stages.