Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments
Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments
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DOI：
10.1029/2005jg000121
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发表时间：
2006-09-19
影响因子：
3.7
通讯作者：
Huang, Yongsong
中科院分区：
文献类型：
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作者：
D'Andrea, William J.;Lage, Melissa;Huang, Yongsong
使用聚合酶链反应（PCR）从来自Sondre Stromfjord地区一组湖泊的滤液、表面沉积物和晚全新世沉积物样本（类似于1000年）中成功扩增了附着藻类的18 S核糖体DNA（rDNA）序列西格陵兰岛。藻类初级生产者的DNA在沉积在寒冷（1 -2摄氏度）、缺氧和硫化物底层水中的层状湖泊沉积物中保存得非常好。系统发育分析的格陵兰附着植物rDNA序列表明，烯酮在格陵兰湖沉积物中产生的附着藻类的类Prymnesiophyceae。来自格陵兰样品的18 S rDNA序列聚类在一个不同的类内，不同于海洋附着植物和以前报道的南极洲Ace湖。在这项研究中的所有样本之间的附着植物rDNA序列的相似性表明，一个单一的烯酮为基础的温度校准可能适用于这些湖泊至少在过去的1000年。这些沉积档案为格陵兰西南部的高分辨率，基于烯酮的古温度重建提供了巨大的希望，格陵兰西南部是一个对北大西洋涛动（NAO）等大气-海洋气候现象敏感的地区。
The 18S ribosomal DNA ( rDNA) sequences of haptophyte algae were successfully amplified using the polymerase chain reaction ( PCR) from water filtrate, surface sediments, and a late-Holocene sediment sample ( similar to 1000 years old) from a group of lakes in the Sondre Stromfjord region of west Greenland. The DNA of the algal primary producer is extremely well preserved in the laminated lake sediments which have been deposited in cold ( 1 degrees-2 degrees C), anoxic, and sulphidic bottom water. Phylogenetic analyses of the Greenland haptophyte rDNA sequences suggest that alkenones in the Greenland lake sediments are produced by haptophyte algae of the class Prymnesiophyceae. The 18S rDNA sequences from the Greenland samples cluster within a distinct phylotype, differing from both marine haptophytes and from those reported previously from Ace Lake, Antarctica. The similarity of haptophyte rDNA sequences among all samples in this study suggests a single alkenone-based temperature calibration may be applied to these lakes for at least the past 1000 years. These sedimentary archives hold great promise for high-resolution, alkenone-based paleotemperature reconstruction of southern west Greenland, a region sensitive to atmospheric-oceanic climate phenomena such as the North Atlantic Oscillation ( NAO).