Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro
Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro
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DOI：
10.1007/s11626-015-9920-0
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发表时间：
2015-10-01
影响因子：
2.1
通讯作者：
Yadav, P. S.
中科院分区：
文献类型：
--
作者：
Ghosh, Kaushalya;Kumar, Rajesh;Yadav, P. S.
最近的研究表明，胎盘羊膜是人类和家畜干细胞的宝贵来源。与其他干细胞来源相比，羊膜的优势包括丰富的可用性，伦理上无异议和非侵入性来源。本研究的目的是从布法罗产后收集的足月胎盘中分离、培养和鉴定羊膜间充质干细胞。我们已经观察到，这两个假定的上皮样和成纤维细胞样细胞培养和维持从足月羊膜。这些细胞在不同代次显示多能性标志物（OCT-4、SOX-2、NANOG、TERT）、间充质干细胞标志物（CD 29、CD 44、CD 105）的阳性表达，而造血标志物（CD 34）基因的阴性表达。此外，这些细胞的碱性磷酸酶染色也呈阳性。任何干细胞的干细胞潜能由其分化成特定细胞类型谱系的潜能决定。在本研究中，我们已经成功地在体外分化成脂肪细胞，软骨细胞和成骨细胞谱系的牙周膜来源的细胞。总之，本研究的结果表明，羊水膜来源的细胞表达多能性和间充质干细胞标志物，并且在体外定向分化后具有分化为间充质谱系细胞类型的细胞的倾向。
Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.