E1A and ras oncogenes synergistically enhance recombinant protein production under control of the cytomegalovirus promoter in BHK-21 cells.
E1A and ras oncogenes synergistically enhance recombinant protein production under control of the cytomegalovirus promoter in BHK-21 cells.
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E1A 和 ras 癌基因在 BHK-21 细胞中巨细胞病毒启动子的控制下协同增强重组蛋白的产生。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
H. Murakami
中科院分区：
文献类型：
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作者：
S. Shirahata;J. Watanabe;K. Teruya;T. Yano;K. Osada;H. Ohashi;H. Tachibana;E. Kim;H. Murakami
在巨细胞病毒立即早期启动子（CMV启动子）的控制下，扩增的ras癌基因在BHK-21细胞中显著提高了重组人白细胞介素-6 （hIL-6）的产生。将腺病毒E1A癌基因进一步转染到上述ras扩增的hIL-6高产BHK细胞中，转染后的产率比未转染的高10倍左右。然而，单独的E1A基因并不能提高生产力。这些结果表明ras和E1A的协同能力可以促进hIL-6的产生。
The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyperproducing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.