Survival of Vibrio fluvialis in seawater under starvation conditions
Survival of Vibrio fluvialis in seawater under starvation conditions
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DOI：
10.1016/j.micres.2006.06.006
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发表时间：
2008-01-01
影响因子：
6.7
通讯作者：
Amina, Bakhrouf
中科院分区：
文献类型：
--
作者：
Amel, Ben Kahla-Nakbi;Amine, Besbes;Amina, Bakhrouf
研究了河流弧菌在有沉积物和无沉积物的海水微宇宙中的生活力。该菌株作为可培养细菌存活至少1年，并且其毒力因子的表达得以维持。在含有沉积物的微宇宙中，河流弧菌的稳定性更高。河流弧菌活的但不可培养的（VBNC）细胞能够复苏到可培养状态长达6年的海洋沉积物中的孵化。这些细胞在海洋肉汤中孵育3个月后恢复了它们最初的生化特性。扩增16S核糖体。DNA（rDNA）限制性内切酶分析（ARDIOR）证实，这是同一株河流弧菌，在所有的微观世界中抵抗了很长一段时间。(c)2006年Elsevier GmbH。All rights reserved.
The viabitity of Vibrio fluvialis in seawater microcosms, with and without sediment was investigated. The strain survived as culturable bacteria for at least 1 year and the expression of its virulence factors was maintained. In microcosms containing sediment Vibrio fluvialis was more stable. Viable but nonculturable (VBNC) cells of Vibrio fluvialis were able to resuscitate to the culturable state up to 6 years of incubation in marine sediment. These cells recuperate their initial biochemical, characteristics after 3 months of incubation in marine broth. Amplified 16S ribosomal. DNA (rDNA) restriction analysis (ARDRA) was used to confirm that it is the same strain of Vibrio fluvialis which resists in all microcosms during a long period of time. (c) 2006 Elsevier GmbH. All rights reserved.