Phenotypic expression of IGF binding protein transcripts in muscle, in vitro and in vivo
Phenotypic expression of IGF binding protein transcripts in muscle, in vitro and in vivo
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DOI：
10.1006/bbrc.2000.2667
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发表时间：
2000-06-24
影响因子：
3.1
通讯作者：
Brownson, C
中科院分区：
文献类型：
--
作者：
Bayol, S;Loughna, PT;Brownson, C
胰岛素样生长因子（IGF-I和ICF-II）是骨骼肌生长的重要组成部分，其作用通过其受体介导，并由六种结合蛋白（IGFBPs）调节。我们研究了IGFBP转录C2 C12细胞培养和成人控制和失神经腓肠肌。在C2 C12细胞中检测到IGFBP-2、-4、-5和-6。在血清减少后1、5和9天，IGFBP-6 mRNA水平保持不变，IGFBP-2水平降低，IGFBP-4和-5升高。在所研究的一系列成人肌肉中，IGFBP-4 mRNA水平相似，IGFBP-5在慢肌和快肌中以不同水平存在，IGFBP-6在胫骨前肌中表达最低。去神经支配导致IGFBP-4和-5转录物的显著上调，但IGFBP-6没有变化。这些结果表明，无论是缺乏神经输入和/或机械负荷，这两者都有助于肌肉萎缩，影响IGFBP的表达。(C)北京大学出版社.
The actions of the insulin-like growth factors (IGF-I and ICF-II) which are essential components of skeletal muscle growth are mediated via their receptors and modulated by six binding proteins (IGFBPs). We studied IGFBP transcripts in C2C12 cell cultures and in adult control and denervated gastrocnemius muscle. IGFBP-2, -4, -5, and -6 were detected in C2C12 cells. IGFBP-6 mRNA levels remained unchanged, IGFBP-2 levels decreased and IGFBP-4 and -5 increased over 1, 5, and 9 days after serum reduction. In a range of adult muscles studied, IGFBP-4 mRNA levels were similar, IGFBP-5 was present at different levels in slow and fast muscles and IGFBP-6 had the lowest expression in the tibialis anterior. Denervation resulted in dramatic up-regulation of IGFBP-4 and -5 transcripts but there was no change in IGFBP-6. These results suggest that either lack of neural input and/or mechanical loading, both of which contribute to muscle atrophy, affect IGFBP expression. (C) 2000 Academic Press.