Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats - similarities to astrocyte grafts
Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats - similarities to astrocyte grafts
复制标题
DOI：
10.1073/pnas.95.7.3908
复制
发表时间：
1998-03-31
影响因子：
11.1
通讯作者：
Prockop, DJ
中科院分区：
文献类型：
--
作者：
Azizi, SA;Stokes, D;Prockop, DJ
神经移植已被用于探索中枢神经系统的发育和帕金森病等疾病组织的修复，在这里，我们检查了直接注射人骨髓基质细胞（MSC）到大鼠大脑中的效果，MSC是来自骨髓的细胞子集，包括远非造血组织的干样前体，通过粘附塑料分离的人MSC被注入纹状体，5至72天后，检查脑切片是否存在供体细胞，大约 20% 的输注细胞已植入，没有炎症反应或排斥的证据，细胞已从注射部位沿着已知的神经干细胞迁移路径迁移到连续的大脑层。输注到大脑后，人类 MSC 失去了对 I 型胶原蛋白抗体的免疫反应性。最初，人类细胞继续用纤连蛋白抗体染色，但纤连蛋白染色区域在 30 天和 72 天时显着减少。结果表明，间充质干细胞可能是多种中枢神经系统疾病的细胞和基因治疗中自体移植的有用载体。
Neurotransplantation has been used 60 explore the development of the central nervous system and for repair of diseased tissue in conditions such as Parkinson's disease, Here, we examine the effects of direct injection into rat brain of human marrow stromal cells (MSCs), a subset of cells from bone marrow that include stem-like precursors far nonhematopoietic tissues, Human MSCs isolated by their adherence to plastic were infused into the corpus striatum, Five to 72 days later, brain sections were examined for the presence of the donor cells, About 20% of the infused cells had engrafted, There was no evidence of an inflammatory response or rejection, The cells had migrated from the injection site along known pathways for migration of neural stem cells to successive lavers of the brain. After infusion into the brain, the human MSCs lost their immunoreactivity to antibodies for collagen I. Initially, the human cells continued to stain with antibodies to fibronectin but the region of staining with fibronectin was significantly decreased at 30 and 72 days. The results suggest that MSCs may be useful vehicles for autotransplantation in both cell and gene therapy for a variety of diseases of the central nervous system.