Characterization of the beta-lactamase promoter of pBR322.
Characterization of the beta-lactamase promoter of pBR322.
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pBR322 β-内酰胺酶启动子的表征。
DOI：
10.1093/nar/9.11.2517
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发表时间：
1981
影响因子：
14.9
通讯作者：
Bennett,GN
中科院分区：
文献类型：
--
作者：
Russell,DR;Bennett,GN
已经使用几种技术表征了源自Tn 3的pBR 322的β-内酰胺酶启动子。转录起始位点位于距离β-内酰胺酶翻译起始密码子35个碱基对处。在体外产生的mRNA具有51 pppGpA末端。结合在这个起始位点的RNA聚合酶保护了大约-50到+20的区域免受DNA酶I的切割。RNA聚合酶快速结合β-内酰胺酶启动子。联想的中场休息时间不到半分钟。解离的半衰期约为6小时。对RNA聚合酶在不同温度下结合的研究表明，在11° C和15°C之间变化很大。这些参数与其他启动子报告的比较进行了讨论。
The β-lactamase promoter of pBR322, derived from Tn3, has been characterized using several techniques. The transcription initiation site islocated 35 base pairs from the translation initiation codon of β-lactamase. The mRNA producedin vitrohas a 51 pppGpA terminus. RNA polymerase bound at this start site protects a region from about −50 to +20 from DNA ase I cleavage using the foot printing technique. RNA polymerase binds rapidly to the (β-lactamase promoter. The half-time of association is less than one-half minute. The half-time of dissociation is approximately 6 hr. A study of the binding of RNA polymerase at different temperatures showed a large change between 11° and 15°C. Comparison of these parameters with those reported for other promoters is discussed.