Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA.
Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA.
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未感染的细胞聚合酶可有效转录早期而非晚期的 1 型单纯疱疹病毒 mRNA。
DOI：
10.1073/pnas.78.10.6139
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发表时间：
1981
影响因子：
11.1
通讯作者：
Wagner,EK
中科院分区：
文献类型：
--
作者：
Frink,RJ;Draper,KG;Wagner,EK
对编码一种早期和一种晚期单纯疱疹病毒1型mRNA 5'端的DNA序列进行了分析，并精确定位了这些mRNA种类的5'端。两种 mRNA 均未剪接，并且 DNA 的非编码链在其各自 5' 端上游包含可识别的 T-A-T-A 和 C-A-T 盒。早期 mRNA 可以通过市售的未感染细胞裂解物系统有效转录，但晚期 mRNA 则不能。早期和晚期 mRNA 之间的这种差异似乎在该病毒中很普遍。
The sequences of the DNAs encoding the 5' ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5' ends of these mRNA species were precisely located. Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5' ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus.