Isolation and characterization of microsatellite loci in the Chinese water snake Enhydris chinensis (Colubridae)
Isolation and characterization of microsatellite loci in the Chinese water snake Enhydris chinensis (Colubridae)
复制标题
中国水蛇EnHydris chinensis（游蛇科）微卫星位点的分离和表征
DOI：
10.1007/s12686-014-0200-1
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发表时间：
2014-04
影响因子：
1.1
通讯作者：
Lin CX
中科院分区：
文献类型：
--
作者：
Du Y;Zheng XX;Yao YT;Lin CX
中国水蛇EnHydris chinensis是一种卵胎生游蛇，分布于中国和越南。它通常用于生产中国蛇油，蛇油以治疗发烧、关节痛和头痛等疾病而闻名。被中国濒危动物红皮书列为易危物种。线粒体DNA和微卫星位点等特定分子标记有助于我们更好地了解蛇的种群结构和进化历史，并对该物种采取有效的保护行动。在这里，我们表征了从 E. chinensis 基因组文库中分离出的八个多态性微卫星位点。从中国江西吉安人群中采集了 47 只个体。这些标记揭示了高度的遗传多样性（每个位点 3-10 个等位基因）和杂合性（HO 范围为 0.532 至 0.957，HE 范围为 0.565 至 0.806）。没有位点表现出与哈代-温伯格平衡的显着偏差。没有证据表明基因座对之间存在连锁不平衡。本论文的研究结果对于今后研究中华沙棘种群结构和遗传多样性具有重要意义。
The Chinese water snake Enhydris chinensis is an ovoviviparous colubrid snake distributed in China and Vietnam. It is commonly used in the production of Chinese snake oil, which is known for treating ailments such as fever, joint pain, and headache. It is listed as a vulnerable species according to the China red data book of endangered animals. Specific molecular markers such as mitochondrial DNA and microsatellite loci are helpful for us to get a better understanding of population structure and evolutionary history of the snake and take effective conservation action for this species. Here, we characterize eight polymorphic microsatellite loci isolated from E. chinensis genomic libraries. Forty-seven individuals were collected from Ji’an population in Jiangxi, China. These markers revealed a high degree of genetic diversity (3–10 alleles per locus) and heterozygosity (HO ranged from 0.532 to 0.957, and HE ranged from 0.565 to 0.806). No locus exhibited significant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. The results of this paper will be useful for future study of population structure and genetic diversity of E. chinensis.