LIPOFUSCIN AUTOFLUORESCENCE - EVIDENCE FOR VITAMIN-A INVOLVEMENT IN THE RETINA
LIPOFUSCIN AUTOFLUORESCENCE - EVIDENCE FOR VITAMIN-A INVOLVEMENT IN THE RETINA
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DOI：
10.1016/0047-6374(87)90088-1
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发表时间：
1987-06-01
影响因子：
5.3
通讯作者：
ROBISON, WG
中科院分区：
文献类型：
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作者：
KATZ, ML;ELDRED, GE;ROBISON, WG
RCS大鼠（一种遗传性视网膜营养不良的品系）退化的感光细胞中出现脂褐素样自发荧光。在具有正常视网膜的动物中，眼内与年龄相关的脂褐素积累仅限于视网膜色素上皮（RPE）。先前的研究已经确定，RPE脂褐素在正常大鼠视网膜中的积累可以通过膳食维生素A缺乏来减少。为了确定RCS大鼠视网膜中光感受器来源的荧光是否与RPE脂褐质荧光有关，研究了饮食维生素A对RCS大鼠视网膜荧光团含量的影响。维生素A缺乏实质上减少了RCS大鼠视网膜感光细胞退化相关的自发荧光。使用薄层色谱法（TLC）从这些视网膜中分离出一种特定的维生素A依赖性荧光团。TLC上的这种荧光团的流动性不同于从正常大鼠的RPE分离的主要年龄依赖性荧光团。因此，如果光感受器和RPE的维生素A依赖性荧光团是相关的，那么似乎光感受器细胞中产生的荧光团一旦被RPE吸收就必须经历化学修饰。RPE和光感受器相关的荧光团都依赖于维生素A，这一事实表明它们之间可能存在这种关系。这些实验表明，RPE与其他脂褐素积累组织有些不同，因为RPE脂褐素荧光团的主要前体来源于另一种细胞类型并通过吞噬作用进入RPE。
A lipofuscin-like autofluorescence develops in the degenerating photoreceptor cells of the RCS rat, a strain with inherited retinal dystrophy. In animals with normal retinas, age-related lipofuscin accumulation in the eye is restricted to the retinal pigment epithelium (RPE). Previous investigations have established that RPE lipofuscin accumulation in the normal rat retina can be reduced by dietary vitamin A deficiency. In order to determine whether the photoreceptor-derived dluorescence in the RCS rat retina is related to RPE lipofuscin fluorescence, the influence of dietary vitamin A on the fluorophore content of the RCS rat retina was studied. Vitamin A deficiency substantially reduced the autofluorescence associated with degenerating photoreceptor cells of the RCS rat retina. A specific vitamin A-dependent fluorophore was isolated from these retinas using thin-layer chromatography (TLC). The mobility of this fluorophore on TLC differs from that of the major age-dependent fluorophore isolated from the RPE of normal rats. Thus, if the vitamin A-dependent fluorophores of the photoreceptors and RPE are related, it appears that the fluorophore generated in the photoreceptor cells must undergo chemical modification once it has been taken up by the RPE. The fact that both the RPE- and photoreceptor-associated fluorophores are vitamin A-dependent suggests that such a relationship between them is likely. These experiments indicate that the RPE is somewhat different from other lipofuscin-accumulating tissues in that a major precursor of RPE lipofuscin fluorophores originates in another cell type and enters the RPE via phagocytosis.