DETECTION OF KLEBSIELLA-PNEUMONIAE AND ESCHERICHIA-COLI STRAINS PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASES
DETECTION OF KLEBSIELLA-PNEUMONIAE AND ESCHERICHIA-COLI STRAINS PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASES
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DOI：
10.1128/jcm.32.3.691-696.1994
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发表时间：
1994-03-01
影响因子：
9.4
通讯作者：
JACOBY, GA
中科院分区：
文献类型：
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作者：
KATSANIS, GP;SPARGO, J;JACOBY, GA
将编码TEM型、SHV型和AmpC家族超广谱β-内酰胺酶的质粒导入常见的大肠埃希菌和肺炎克雷伯菌宿主中，建立一个同质评价板，用于评估5种测试系统检测8种β-内酰胺类抗生素的能力。尽管琼脂稀释法或纸片扩散法测得的MIC值增加，纸片扩散区直径减小，但往往达不到耐药的临界点，而且这两种方法在检测对羟亚胺-β-内酰胺类抗生素的耐药性时都不敏感。MicroScan 18-h微量稀释法或VITEK快速自动化程序同样不敏感。头孢他啶是检测产超广谱β-内酰胺酶的最佳单项试验抗生素。β-内酰胺酶Tm-7和Tm-12尤其难以检测。由于这些困难，超广谱β-内酰胺酶的流行可能比目前所认识到的更大。
Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for evaluating the abilities of five test systems to detect resistance to eight beta-lactam antibiotics. Although MICs, as determined by agar dilution or E test strips, were increased and disk diffusion zone diameters were diminished, breakpoints for resistance were often not reached, and neither approach was sensitive in detecting resistance to oxyimino-beta-lactams. The MicroScan 18-h microdilution or Vitek rapid automated procedures were similarly insensitive. Ceftazidime was the best single test antibiotic for detecting extended-spectrum beta-lactamase production. beta-Lactamases TEM-7 and TEM-12 were particularly difficult to detect. Because of such difficulties, the prevalence of extended-spectrum beta-lactamases is likely to be greater than is currently appreciated.