Guided Implant Surgery: What Is the Influence of This New Technique on Bone Cell Viability?
Guided Implant Surgery: What Is the Influence of This New Technique on Bone Cell Viability?
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DOI：
10.1016/j.joms.2012.10.017
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发表时间：
2013-03-01
影响因子：
1.9
通讯作者：
Garcia Junior, Idelmo Rangel
中科院分区：
文献类型：
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作者：
dos Santos, Pamela Leticia;Queiroz, Thallita Pereira;Garcia Junior, Idelmo Rangel
目的：评价种植体截骨术对即刻骨细胞活性的影响，比较引导手术种植体放置与经典的钻孔procedure.Materials和方法：对于这项研究，20只兔子被使用。将动物分为引导手术组（GG）和对照组（CG），然后分为4个亚组-亚组1、2、3和4-分别对应于使用10、20、30和40次的演习。所有动物的每根胫骨均接受5次截骨术，其中一根胫骨采用经典钻孔手术，另一根胫骨采用引导手术。结果：免疫组化显示，骨钙素（osteocalcin，OPG）、核因子κ B配体受体激活因子（receptor activator of nuclear factor kappa，RANKL）、caspase 3在对照组中表达明显增高，在再次使用骨钻后，其表达水平基本不变。尽管GG和CG的RANKL和OPG表达无统计学差异，但GG的RANKL/OPG比值倾向于更高。此外，半胱天冬酶3的表达升高的GG，成比例的截骨术的数量，表明在GG的凋亡指数增加。结论：经典的钻孔程序是更有利于细胞活力比引导手术。(C)2013年美国口腔颌面外科医师协会J Oral Maxillofac Surg 71：505-512，2013
Purpose: To evaluate the effect of implant osteotomy on immediate bone cell viability, comparing guided surgery for implant placement with the classic drilling procedure.Materials and Methods: For this study, 20 rabbits were used. The animals were divided into a guided surgery group (GG) and a control group (CG) and were then divided into 4 subgroups-subgroups 1, 2, 3, and 4-corresponding to drills used 10, 20, 30, and 40 times, respectively. All animals received 5 osteotomies in each tibia, by use of the classic drilling procedure in one tibia and guided surgery in the other tibia. The osteotomized areas were removed and processed immunohistochemically for detection of osteocalcin, receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and caspase 3.Results: Immunohistochemical analysis showed that osteocalcin expression was initially higher in the CG and remained constant after drill reutilization. Although the expressions of RANKL and OPG were not statistically different for the GG and CG, the RANKL/OPG ratio tended to be higher for the GG. Moreover, caspase 3 expression was elevated in the GG, proportionally to the number of osteotomies, indicating an increase in the apoptosis index in the GG.Conclusions: The classic drilling procedure is more favorable to cell viability than guided surgery. (C) 2013 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 71:505-512, 2013