Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat
Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat
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DOI：
10.1007/s00122-022-04184-y
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发表时间：
2022-07
影响因子：
5.4
通讯作者：
Hao Jiang;Yu Fang;Dong Yan;Sihan Liu;Jun Wei;Fei-Long Guo;Xing-ting Wu;H. Cao;Chang-Bin Yin
中科院分区：
文献类型：
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作者：
Hao Jiang;Yu Fang;Dong Yan;Sihan Liu;Jun Wei;Fei-Long Guo;Xing-ting Wu;H. Cao;Chang-Bin Yin
通过关联分析，确定了12个与小麦穗发芽耐性相关的QTL。利用农杆菌介导法对两个标记进行了验证，并验证了Qgpf.cas-3B2的候选基因TaNAC074。由于这一威胁，育种工作者必须识别数量性状基因座(QTL)和与PHS耐性相关的基因(PHST)，以减少PHS对种子低休眠造成的负面影响。在这项研究中，我们评估了一组302个不同的小麦基因型在四个环境中的PHST，并用高密度小麦660kSNP阵列对该小组进行了基因分型。通过全基因组关联研究，我们确定了12个与PHST显著相关的稳定基因座(P<； 0.0001)，解释了3.34个 − 9.88%的表型变异。其中有7个基因座与已报道的QTL和基因位于同一位置。5个基因座(Qgpf.cas-3B.2、Qgpf.cas-3B.3、Qgpf.cas-3B.4、Qgpf.cas-7B.2和Qgpf.cas-7B.3)可能是与PHST相关的新的QTL。此外，还为Qgpf.cas-3A和Qgpf.cas-7B.3开发了两个分子标记，并用不同的233份小麦材料进行了验证。最后，通过对233份小麦材料的CAPS(裂解扩增多态序列)标记关联分析以及转基因小麦的表达和表型分析，证实了Qgpf.cas-3B2候选基因TaNAC074的PHST相关功能。过表达TaNAC074显著降低了小麦种子的休眠。本研究有助于拓宽PHST的遗传基础和分子标记辅助育种。
Key messageTwelve QTL associated with pre-harvest sprouting tolerance were identified using association analysis in wheat. Two markers were validated and a candidate geneTaNAC074forQgpf.cas-3B.2was verified usingAgrobacterium-mediated transformation.AbstractPre-harvest sprouting (PHS) is a considerable global threat to wheat yield and quality. Due to this threat, breeders must identify quantitative trait loci (QTL) and genes conferring PHS-tolerance (PHST) to reduce the negative effects of PHS caused by low seed dormancy. In this study, we evaluated a panel of 302 diverse wheat genotypes for PHST in four environments and genotyped the panel with a high-density wheat 660 K SNP array. By using a genome-wide association study (GWAS), we identified 12 stable loci significantly associated with PHST (P< 0.0001), explaining 3.34 − 9.88% of the phenotypic variances. Seven of these loci co-located with QTL and genes reported previously. Five loci (Qgpf.cas-3B.2,Qgpf.cas-3B.3,Qgpf.cas-3B.4,Qgpf.cas-7B.2, andQgpf.cas-7B.3), located in genomic regions with no known PHST QTL or genes, are likely to be new QTL conferring PHST. Additionally, two molecular markers were developed forQgpf.cas-3AandQgpf.cas-7B.3, and validated using a different set of 233 wheat accessions. Finally, the PHST-related function of candidate geneTaNAC074forQgpf.cas-3B.2was confirmed by CAPS (cleaved amplified polymorphic sequences) marker association analysis in 233 wheat accessions and by expression and phenotypic analysis of transgenic wheat. Overexpression ofTaNAC074significantly reduced seed dormancy in wheat. This study contributes to broaden the genetic basis and molecular marker-assisted breeding of PHST.